Factors affecting 2‐hydroxypropiophenone formation by benzoylformate decarboxylase from Pseudomonas putida
Factors affecting 2‐hydroxypropiophenone formation by benzoylformate decarboxylase from Pseudomonas putida
复制标题
恶臭假单胞菌苯甲酰甲酸脱羧酶生成 2-羟基苯丙酮的影响因素
DOI：
10.1002/bit.260391010
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发表时间：
1992
影响因子：
3.8
通讯作者：
O. Ward
中科院分区：
文献类型：
--
作者：
R. Wilcocks;O. Ward
在1.6 M乙醛存在下，通过恶臭假单胞菌ATCC 12633的酶提取物将苯甲酰甲酸酯（100 mM）定量转化为偶姻化合物、2-羟基苯丙酮（61.76 mM）和苯甲醛（38.2 mM）。在pH 6.0和30°C下进行生物转化，孵育时间为60 min。在生物转化混合物中，偶姻形成酶苯甲酰甲酸脱羧酶的活性为1.23单位/mL。偶姻形成随着pH在4-5范围内显著增加，并且在pH 5-8范围内具有宽的活性平台。在20-40°C的范围内也观察到偶姻形成的宽的最佳温度。
Benzoylformate (100 mM) was quantitatively converted to the acyloin compound, 2‐hydroxypropiophenone (61.76 mM) and benzaldehyde (38.2 mM) by an enzyme extract from Pseudomonas putida ATCC 12633 in the presence of 1.6M acetaldehyde. Biotransformations were carried out at pH 6.0 and 30°C with an incubation time of 60 min. Activity of the acyloin forming enzyme, benzoylformate decarboxylase, was 1.23 units/mL in the biotransformation mixture. Acyloin formation increased dramatically with pH in the range 4–5 and had a broad activity plateau in the pH range 5–8. A broad temperature optimum for acyloin formation was also observed in the range 20–40°C.