The impact of low levels of collagen IX and pyridinoline on the mechanical properties of in vitro engineered cartilage
The impact of low levels of collagen IX and pyridinoline on the mechanical properties of in vitro engineered cartilage
复制标题
低水平的 IX 胶原蛋白和吡啶啉对体外工程软骨机械性能的影响
DOI：
10.1016/j.biomaterials.2008.10.042
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发表时间：
2009-02-01
期刊：
影响因子：
14
通讯作者：
Cao, Yilin
中科院分区：
文献类型：
--
作者：
Yan, Dan;Zhou, Guangdong;Cao, Yilin
体外工程化软骨的应用已成为修复软骨缺损的一种很有前途的方法。然而，体外工程化软骨的机械性能较差，限制了其临床应用的潜力。研究表明，细胞外基质（extracellular matrix，ECM）成分与工程化软骨的力学强度密切相关，但目前尚不清楚哪些成分在决定工程化软骨力学性能方面起关键作用。为了解决这个问题，进行了天然软骨、体内和体外工程化软骨中软骨特异性组分的定量分析，并进一步分析了各种ECM分子与杨氏模量之间的相关性。结果表明，许多ECM分子，如高度硫酸化的糖胺聚糖（GAG），胶原蛋白II，IX和吡啶啉（PYR），有助于软骨的机械强度。体外工程化软骨和应力刺激的体外工程化软骨之间的进一步比较，已知具有更强的机械性能，表明只有胶原IX和PYR，而不是GAG和胶原II，是决定体外工程化软骨的机械性能的关键因素。这些结果表明，体外环境缺乏增强胶原交联的生态位，所述胶原交联在软骨形成期间由胶原IX和PYR介导。因此，这一发现为未来通过提高这两种分子的水平来体外工程化强壮的软骨提供了线索。(C)2008爱思唯尔有限公司保留所有权利。
The application of in vitro engineered cartilage has become a promising approach to repair cartilage defects. Nevertheless, the poor mechanical properties of in vitro engineered cartilage limit its potential for clinical applications. Studies have shown that the extracellular matrix (ECM) components are strongly correlated with the mechanical strength of engineered cartilage, but it remains unclear which components play a key role in determining the mechanical property of engineered cartilage. To address this issue, quantitative analyses of cartilage-specific components among native cartilage, in vivo and in vitro engineered cartilages were performed, and the correlation between various ECM molecules and Young's modulus was further analyzed. The results showed that many ECM molecules, such as highly sulphated glycosaminoglycan (GAG), collagens II, IX, and pyridinoline (PYR), contributed to the mechanical strength of cartilages. Further comparison between in vitro engineered cartilage and stress-stimulated in vitro engineered cartilage, known to have stronger mechanical properties, showed that only collagen IX and PYR, but not GAG and collagen II, were the key factors determining the mechanical properties of in vitro engineered cartilage. These results indicate that in vitro environment lacks the niche for enhancing collagen crosslinking that is mediated by collagen IX and PYR during cartilage formation. Thus, the discovery provides a clue for engineering strong cartilage in vitro in the future by enhancing the levels of these two molecules. (C) 2008 Elsevier Ltd. All rights reserved.