Aligned PLGA/HA nanofibrous nanocomposite scaffolds for bone tissue engineering
Aligned PLGA/HA nanofibrous nanocomposite scaffolds for bone tissue engineering
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DOI：
10.1016/j.actbio.2008.07.019
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发表时间：
2009-01-01
影响因子：
9.7
通讯作者：
Nyalro, Elijah
中科院分区：
文献类型：
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作者：
Jose, Moncy V.;Thomas, Vinoy;Nyalro, Elijah
以聚(D，L-丙交酯-乙交酯)(PLGA)和纳米羟基磷灰石(Nano-HA)为基质，采用静电纺丝法制备骨组织工程取向纳米纤维支架。扫描电子显微镜表征表明，不同含量的纳米HA(1、5、10和20wt.%)的加入使纤维的平均直径从300 nm(整齐的PLGA)增加到700 nm(20%的纳米HA)。在较高浓度下(~gt；=10%)，观察到HA的团聚现象，这在浓度为20%时有显著影响，纳米HA的存在导致纤维断裂。热分析表明，电纺的快速处理锁定了PLGA的非晶态，导致支架的玻璃化转变温度降低。此外，随着纳米羟基磷灰石浓度的增加，其玻璃化转变温度也随之升高。支架的动态力学行为反映了形态观察，即纳米羟基磷灰石在较低浓度(1%和5%)时起增强作用，但在较高浓度(10%和20%)时起到缺陷作用。纳米羟基磷灰石的储能模量值从纯PLGA的441 Mpa增加到5%的724 Mpa，但随着浓度的增加，材料的储能模量值下降，20%的纳米Ha材料的储能模量值为371 Mpa。降解特性表明，亲水性纳米羟基磷灰石对磷酸盐缓冲盐水的吸收和质量损失有影响。力学行为呈现正弦变化趋势，由于介质的塑化作用，弹性模量在第一周略有下降，随后由于收缩而增加，随后由于降解而在第六周下降。(C)2008 Acta Materialia Inc.由爱思唯尔有限公司出版。版权所有。
Aligned nanofibrous scaffolds based on poly(D,L-lactide-co-glycolide) (PLGA) and nano-hydroxyapatite (nano-HA) were synthesized by electrospinning for bone tissue engineering. Morphological characterization using scanning electron microscopy showed that the addition of different amounts of nano-HA (1, 5, 10 and 20 wt.%) increased the average fiber diameter from 300 nm (neat PLGA) to 700 nm (20% nano-HA). At higher concentrations (>= 10%), agglomeration of HA was observed and this had a marked effect at 20% concentration whereby the presence of nano-HA resulted in fiber breaking. Thermal characterization showed that the fast processing of electrospinning locked in the amorphous character of PLGA,this resulted in a decrease in the glass transition temperature of the scaffolds. Furthermore, an increase in the glass transition temperature was observed with increasing nano-HA concentration. The dynamic mechanical behavior of the scaffolds reflected the morphological observation, whereby nano-HA acted as reinforcements at lower concentrations (1% and 5%) but acted as defects at higher concentrations (10% and 20%). The storage modulus value of the scaffolds increased from 441 MPa for neat PLGA to 724 MPa for 5% nano-HA; however, further increasing the concentration leads to a decrease in storage modulus, to 371 MPa for 20% nano-HA. Degradation characteristics showed that hydrophilic nano-HA influenced phosphate-buffered saline uptake and mass loss. The mechanical behavior showed a sinusoidal trend with a slight decrease in modulus by week I due to the plasticizing effect of the medium followed by an increase due to shrinkage, and a subsequent drop by week 6 due to degradation. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.