A SILVER STAIN FOR THE RAPID QUANTITATIVE DETECTION OF PROTEINS OR NUCLEIC-ACIDS ON MEMBRANES OR THIN-LAYER PLATES
A SILVER STAIN FOR THE RAPID QUANTITATIVE DETECTION OF PROTEINS OR NUCLEIC-ACIDS ON MEMBRANES OR THIN-LAYER PLATES
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DOI：
10.1016/0003-2697(86)90160-0
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发表时间：
1986-07-01
影响因子：
2.9
通讯作者：
PRATT, ME
中科院分区：
文献类型：
--
作者：
MERRIL, CR;PRATT, ME
一个相对简单的银染色，它需要不到15分钟的执行已被开发用于检测纳克数量的蛋白质和DNA的纤维素膜和薄层板。该染色证明了银密度与蛋白质或DNA的量之间的可再现曲线关系，蛋白质的平均浓度范围为1至300 ng，DNA的平均浓度范围为10至710 ng。膜上的蛋白质和DNA易于染色，结合染色的灵敏度和再现性，允许使用该程序定量测定纳克量的蛋白质和DNA。这种银染法的简单性也使我们能够对单个氨基酸、嘌呤和嘧啶碱基、核苷、均聚物和已知序列的小肽的染色特性进行研究。这一调查表明，碱性氨基酸，特别是赖氨酸和组氨酸，以及含硫氨基酸在蛋白质检测中的重要性。嘌呤碱基在DNA检测中可能起重要作用。
A relatively simple silver stain which takes less than 15 min to perform has been developed for the detection of nanogram quantities of proteins and DNA on cellulose membranes and thin layer plates. This stain demonstrates a reproducible curvilinear relationship between silver density and the amount of protein or DNA, over an averaged concentration range from 1 to 300 ng for proteins and 10 to 710 ng for DNA. The ease of staining proteins and DNA on membranes, combined with the stain''s sensitivity and reproducibility, permits the use of this procedure for the quantitative determination of nanogram amounts of proteins and DNA. The simplicity of this silver stain has also permitted a survey of the staining properties of indiviudal amino acids,purine and pyrimidine bases, nucleosides, homopolymers, and small peptides of known sequence. This survey demonstrated the importance of the basic amino acids, particularly lysine and histidine, and the sulfur-containing amino acids in the detection of proteins. It also indicated that the purine bases may play an important role in the detection of DNA.