DNaseY: A rat DNaseI-like gene coding for a constitutively expressed chromatin-bound endonuclease
DNaseY: A rat DNaseI-like gene coding for a constitutively expressed chromatin-bound endonuclease
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DOI：
10.1021/bi9800597
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发表时间：
1998-07-14
期刊：
影响因子：
2.9
通讯作者：
Sikorska, M
中科院分区：
文献类型：
--
作者：
Liu, QY;Pandey, S;Sikorska, M
一个大鼠基因，命名为DNaseY，编码一个36 kDa的核酸内切酶进行了鉴定和克隆。cDNA序列分析表明它是人DNAS1L3的大鼠同源物。DNaseY基因产物与DNaseI具有42%的同一性，包括保守的关键活性位点残基、必需的二硫键、钙结合结构域和信号肽，以及3个签名框中的2个。值得注意的是，DNaseY有2个核定位信号，并且比DNaseI（pI 4.8）碱性更强（pI 9.5）。DNaseY基因含有许多与DNaseI类似的外显子，由更大的内含子分隔，导致与DNaseI相比基因> 17 kb。
A rat gene, designated DNaseY, encoding a 36 kDa endonuclease was identified and cloned. Sequence analysis of the cDNA showed it to be the rat homologue of human DNAS1L3. The DNaseY gene product had 42% identity to DNaseI, including conserved critical active site residues, the essential disulfide bridge, the calcium binding domain, and a signal peptide, as well as 2 of the 3 signature boxes. Significantly, DNaseY had 2 nuclear localization signals and was more basic (pI 9.5) than DNaseI (pI 4.8). The DNaseY gene contained a number of exons similar to that of DNaseI, separated by much larger introns, resulting in a gene of >17 kb compared to