Specific roles of the αVβ1, αVβ3 and αVβ5 integrins in avian neural crest cell adhesion and migration on vitronectin
Specific roles of the αVβ1, αVβ3 and αVβ5 integrins in avian neural crest cell adhesion and migration on vitronectin
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发表时间：
1994-09
期刊：
影响因子：
4.6
通讯作者：
M. Delannet;F. Martin;B. Bossy;D. Cheresh;L. F. Reichardt;J. Duband
中科院分区：
文献类型：
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作者：
M. Delannet;F. Martin;B. Bossy;D. Cheresh;L. F. Reichardt;J. Duband
为了确定潜在的重要细胞外基质粘附分子在神经嵴细胞迁移，玻连蛋白及其相应的整合素受体的可能作用进行了检查，在禽神经嵴细胞的粘附和迁移在体外。玻连蛋白上的粘附和迁移与纤连蛋白上发现的粘附和迁移相当，并且可以通过抗玻连蛋白抗体和通过RGD肽几乎完全消除。免疫沉淀和免疫细胞化学分析显示，神经嵴细胞主要表达α V β 1，α V β 3和α V β 5整合素作为可能的玻连蛋白受体。用功能干扰抗体进行的细胞粘附和迁移抑制试验表明，神经嵴细胞与玻连蛋白的粘附基本上由一种或多种不同的α V整合素介导，α V β 1可能占优势，而细胞迁移主要涉及α V β 3和α V β 5整合素。免疫荧光标记培养的运动神经嵴细胞显示，α V整合素的差异分布在细胞表面上。β 1和α V亚基都弥漫在细胞表面和粘着斑部位，与黏着斑蛋白，talin和α-辅肌动蛋白，而α V β 3和α V β 5整合素基本上弥漫在细胞表面。最后，玻连蛋白可以检测到免疫印迹和免疫组织化学在早期胚胎的神经嵴的个体发育。特别是与迁移的神经嵴细胞的表面密切相关。总之，我们的研究表明，神经嵴细胞可以粘附和迁移玻连蛋白在体外的RGD依赖性机制，涉及至少α V β 1，α V β 3和α V β 5整合素，这些整合素可能有特定的作用，在控制细胞粘附和迁移。
To identify potentially important extracellular matrix adhesive molecules in neural crest cell migration, the possible role of vitronectin and its corresponding integrin receptors was examined in the adhesion and migration of avian neural crest cells in vitro. Adhesion and migration on vitronectin were comparable to those found on fibronectin and could be almost entirely abolished by antibodies against vitronectin and by RGD peptides. Immunoprecipitation and immunocytochemistry analyses revealed that neural crest cells expressed primarily the alpha V beta 1, alpha V beta 3 and alpha V beta 5 integrins as possible vitronectin receptors. Inhibition assays of cellular adhesion and migration with function-perturbing antibodies demonstrated that adhesion of neural crest cells to vitronectin was mediated essentially by one or more of the different alpha V integrins, with a possible preeminence of alpha V beta 1, whereas cell migration involved mostly the alpha V beta 3 and alpha V beta 5 integrins. Immunofluorescence labeling of cultured motile neural crest cells revealed that the alpha V integrins are differentially distributed on the cell surface. The beta 1 and alpha V subunits were both diffuse on the surface of cells and in focal adhesion sites in association with vinculin, talin and alpha-actinin, whereas the alpha V beta 3 and alpha V beta 5 integrins were essentially diffuse on the cell surface. Finally, vitronectin could be detected by immunoblotting and immunohistochemistry in the early embryo during the ontogeny of the neural crest. It was in particular closely associated with the surface of migrating neural crest cells. In conclusion, our study indicates that neural crest cells can adhere to and migrate on vitronectin in vitro by an RGD-dependent mechanism involving at least the alpha V beta 1, alpha V beta 3 and alpha V beta 5 integrins and that these integrins may have specific roles in the control of cell adhesion and migration.