Infection of neuroretinal cells in vitro by avian sarcoma viruses UR1 and UR2: transformation, cell growth stimulation, and changes in transducin levels.
Infection of neuroretinal cells in vitro by avian sarcoma viruses UR1 and UR2: transformation, cell growth stimulation, and changes in transducin levels.
复制标题
禽肉瘤病毒 UR1 和 UR2 体外感染神经视网膜细胞：转化、细胞生长刺激和转导蛋白水平的变化。
DOI：
10.1016/0042-6822(87)90023-7
复制
发表时间：
1987
期刊：
影响因子：
3.7
通讯作者：
Balduzzi,PC
中科院分区：
文献类型：
--
作者：
Notter,MF;Navon,SE;Fung,BK;Balduzzi,PC
用禽肉瘤病毒 UR1 和 UR2 体外感染分化的鸡胚神经视网膜细胞，导致支持细胞和神经元细胞的有丝分裂刺激和形态转变。这通过转化细胞连续增殖超过 4 个月以及重新聚集的菌落在液体培养基和软琼脂中的生长来证明。根据体外激酶活性测定判断，UR1和UR2的转化蛋白p150gag-fps和p68gag-ros的产生分别与转化的鸡胚成纤维细胞的相似。在许多动物物种的视网膜中发现的 GTP 结合蛋白转导蛋白的两个蛋白亚基 Tβ 和 Tγ，但不存在 Tα，存在于对照神经视网膜细胞中。劳斯肉瘤病毒或 UR2 感染导致 Tγ 合成受到抑制，Tβ 样蛋白产生增强。
Infectionin vitroof differentiating chick embryo neuroretinal cells with avian sarcoma viruses UR1and UR2results in mitogenic stimulation and morphologic conversion of both support and neuronal cells. This was shown by the continuous propagation of transformed cells for over 4 months and growth of reaggregated colonies in liquid medium as well as in soft agar. Production of the transforming proteins p150gag-fpsand p68gag-rosof UR1and UR2, respectively, was similar to that of transformed chick embryo fibroblasts, as judged fromin vitrokinase activity assays. The two protein subunits, Tβand Tγ, but not Tαof the GTP binding protein transducin, found in the retina of many animal species, were present in control neuroretinal cells. Infection with Rous sarcoma virus or UR2resulted in an inhibition of Tγsynthesis and enhancement of Tβ-like protein production.