Basic proline-rich proteins from human parotid saliva: complete covalent structure of protein IB-9 and partial structure of protein IB-6, members of a polymorphic pair.
Basic proline-rich proteins from human parotid saliva: complete covalent structure of protein IB-9 and partial structure of protein IB-6, members of a polymorphic pair.
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来自人腮腺唾液的碱性富含脯氨酸的蛋白质：蛋白质 IB-9 的完整共价结构和蛋白质 IB-6 的部分结构，多态性对的成员。
DOI：
10.1021/bi00268a036
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Keller,P
中科院分区：
文献类型：
--
作者：
Kauffman,D;Wong,R;Bennick,A;Keller,P
材料与方法氯条蛋白酶和胰蛋白酶（处理过的TPCK1）来自Worthington公司，2倍结晶的木瓜蛋白酶悬浮液来自Sigma公司，2倍结晶的弹性蛋白酶悬浮液来自Mann研究实验室。丹酰氯、丹酰氨基酸、PITC和F3CCOOH分别从Gallard-Schlesinger化学公司的Pierce和Cheng Chin聚酰胺片中获得。如前所述，IB-9是由单个人的腮腺唾液制备的（Kauffman & Keller, 1979）。b -9的梭菌酶切。将10 mg IB-9在2 mL 0.05 M碳酸氢铵（pH 8，含0.27 M）溶液中，37℃条件下酶切2 h
Materials and MethodsClostripain and trypsin (TPCK1 treated) were obtained from Worthington, papain suspension 2 times crystallized was from Sigma, and elastase suspension 2 times crystallized was from Mann Research Laboratories. Dansyl chloride, dansyl amino acids, PITC, and F3CCOOH were obtained from Pierce and Cheng Chin polyamide sheets from Gallard-Schlesinger Chemical Corp.Isolation of Salivary Protein IB-9. IB-9 was prepared from human parotid saliva of a single individual as previously de-scribed (Kauffman & Keller, 1979). Clostripain Digestion of IB-9. Clostripain digestion of 10 mg of IB-9 was carried out at 37 C for 2 h in 2 mL of 0.05 M ammonium hydrogen carbonate, pH 8, containing 0.27 M