A Controlled Study on the Characterisation of Bioaerosols Emissions from Compost
A Controlled Study on the Characterisation of Bioaerosols Emissions from Compost
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DOI：
10.3390/atmos9100379
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发表时间：
2018-09
期刊：
影响因子：
2.9
通讯作者：
Z. Nasir;C. Rolph;S. Collins;D. Stevenson;T. Gladding;E. Hayes;Ben Williams;S. Khera;S. Jackson-S
中科院分区：
文献类型：
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作者：
Z. Nasir;C. Rolph;S. Collins;D. Stevenson;T. Gladding;E. Hayes;Ben Williams;S. Khera;S. Jackson-S
生物废物处理过程中产生的生物气溶胶排放是公众关注的问题。为了更好地分解生物气溶胶，并评估一系列的测量方法，我们在受控条件下雾化绿色废物堆肥。同时部署了可行和不可行的Andersen采样器、旋风采样器和真实的时间生物气溶胶检测系统（光谱强度生物气溶胶传感器（SIBS））。对于低排放和高排放情景，荧光颗粒的数量加权分数在所有颗粒的22-26%的范围内。通过SIBS观察到的总体荧光光谱分布在298至735 nm的16个波长带上表现出几个峰。粒度分级的内毒素图谱显示，大多数内毒素存在于2.1-9 μm的空气动力学直径级分中，尽管在更细的粒度级分中发现了高达27%的内毒素。通过培养、基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）和定量聚合酶链反应（qPCR）检测了一系列微生物，包括嗜肺军团菌血清群1。这些发现有助于我们了解堆肥场生物气溶胶的物理化学和生物学特性，并为未来的生物气溶胶测量提供监测方法和数据解释。
Bioaerosol emissions arising from biowaste treatment are an issue of public concern. To better characterise the bioaerosols, and to assess a range of measurement methods, we aerosolised green waste compost under controlled conditions. Viable and non-viable Andersen samplers, cyclone samplers and a real time bioaerosol detection system (Spectral Intensity Bioaerosol Sensor (SIBS)) were deployed simultaneously. The number-weighted fraction of fluorescent particles was in the range 22–26% of all particles for low and high emission scenarios. Overall fluorescence spectral profiles seen by the SIBS exhibited several peaks across the 16 wavelength bands from 298 to 735 nm. The size-fractionated endotoxin profile showed most endotoxin resided in the 2.1–9 μm aerodynamic diameter fraction, though up to 27% was found in a finer size fraction. A range of microorganisms were detected through culture, Matrix Assisted Laser Desorption and Ionisation Time of Flight Mass Spectrometry (MALDI-TOF) and quantitative polymerase chain reaction (qPCR), including Legionella pneumophila serogroup 1. These findings contribute to our knowledge of the physico-chemical and biological characteristics of bioaerosols from composting sites, as well as informing future monitoring approaches and data interpretation for bioaerosol measurement.