Development of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection and identification of Campylobacter jejuni, Campylobacter coli and Campylobacter fetus
Development of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection and identification of Campylobacter jejuni, Campylobacter coli and Campylobacter fetus
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DOI：
10.1111/j.1574-695x.2007.00369.x
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发表时间：
2008-03-01
影响因子：
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通讯作者：
Yamasaki, Shinji
中科院分区：
文献类型：
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作者：
Asakura, Masahiro;Samosornsuk, Worada;Yamasaki, Shinji
建立了一种基于细胞致死性膨胀毒素（cdt）基因的种特异性多重PCR方法，分别检测空肠弯曲菌、结肠弯曲菌和胎儿弯曲菌的cdtA、cdtB和cdtC基因，并对7个种的76株弯曲菌和8个属的131株其他细菌进行了检测。C. jejuni、C. coli或C.胎儿，分别可以成功地扩增使用相应的一套引物在一个高度物种特异性的方式。此外，特定物种的cdtA、cdtB或cdtC基因的特异性引物组可以从两个或所有三个物种的DNA模板的混合物中扩增所需基因。C. jejuni、C. coli或C.根据cdtA、cdtB或cdtC基因的存在，通过多重PCR测定胎儿为10-100 CFU试管（-1）。这些数据表明，cdt基因为基础的多重PCR检测可能是有用的，快速，准确的检测，以及在一个物种特异性的方式来鉴定弯曲杆菌菌株。
A cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection of cdtA, cdtB or cdtC gene of Campylobacter jejuni, Campylobacter coli or Campylobacter fetus, respectively, was developed and evaluated with 76 Campylobacter strains belonging to seven different species and 131 other bacterial strains of eight different genera. The cdtA, cdtB or cdtC gene of C. jejuni, C. coli or C. fetus, respectively, could be successfully amplified using the corresponding set of primers in a highly species-specific manner. Furthermore, the specific primer set for the cdtA, cdtB or cdtC gene of a particular species could amplify the desired gene from a mixture of DNA templates of any of two or all three species. The detection limit of C. jejuni, C. coli or C. fetus was 10-100 CFU tube(-1) by the multiplex PCR assay on the basis of the presence of the cdtA, cdtB or cdtC gene. These data indicate that the cdt gene-based multiplex PCR assay may be useful for rapid and accurate detection as well as identification of Campylobacter strains in a species-specific manner.