Sequences of the recA gene and protein.
Sequences of the recA gene and protein.
复制标题
recA 基因和蛋白质的序列。
DOI：
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发表时间：
1980
影响因子：
11.1
通讯作者：
W. Rupp
中科院分区：
文献类型：
--
作者：
A. Sancar;C. Stachelek;W. Konigsberg;W. Rupp
We have determined the nucleotide sequence of the recA gene of Escherichia coli; this permits the formulation of the primary structure for the recA protein. This structure is consistent with the amino acid composition of the tryptic peptides obtained from the recA protein. The coding region of the recA gene has 1059 base pairs, which specify 352 amino acids. The recA protein has alanine and phenylalanine as its NH2- and COOH-terminal amino acids, respectively, and has the following amino acid composition: Cys3 Asp20 Asn15 Met9 Thr17 Ser20 Glu30 Gln13 Pro10 Gly35 Ala38 Val22 Ile27 Leu31 Tyr7 Phe10 His2Lys27 Trp2 Arg14. Of the three cysteine residues, only two can be alkylated under reducing and denaturing conditions. The molecular weight of the recA polypeptide is 37,842.
