High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor.
High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor.
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DOI：
10.1126/science.aab0810
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发表时间：
2015-12-11
期刊：
影响因子：
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通讯作者：
Schnitzer MJ
中科院分区：
文献类型：
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作者：
Gong Y;Huang C;Li JZ;Grewe BF;Zhang Y;Eismann S;Schnitzer MJ
遗传编码电压指示器（GEVI）是一种很有前途的毫秒级神经元动力学荧光读出技术。以前的GEVI信号传导速度和动态范围不足，无法解析活体动物的动作电位。我们通过共振能量转移将来自视紫红质蛋白的快速电压敏感域耦合到明亮的荧光团。由此产生的GEVI是足够明亮和快速的报告神经元动作电位和膜电压动态在清醒的小鼠和苍蝇，解决快速尖峰列车与0.2毫秒的定时精度在尖峰检测错误率的数量级优于以前的GEVI。在体内成像显示感觉诱发的反应，包括躯体尖峰，树突动力学，和细胞内电压传播。这些结果使神经元电生理学和编码的体内光学研究成为可能，并激励高速显微镜的进一步发展。
Genetically encoded voltage indicators (GEVIs) are a promising technology for fluorescence readout of millisecond-scale neuronal dynamics. Previous GEVIs had insufficient signaling speed and dynamic range to resolve action potentials in live animals. We coupled fast voltage-sensing domains from a rhodopsin protein to bright fluorophores through resonance energy transfer. The resulting GEVIs are sufficiently bright and fast to report neuronal action potentials and membrane voltage dynamics in awake mice and flies, resolving fast spike trains with 0.2-millisecond timing precision at spike detection error rates orders of magnitude better than previous GEVIs. In vivo imaging revealed sensory-evoked responses, including somatic spiking, dendritic dynamics, and intracellular voltage propagation. These results empower in vivo optical studies of neuronal electrophysiology and coding and motivate further advancements in high-speed microscopy.