Effect of Gold Nanoparticle Aggregation on Cell Uptake and Toxicity
Effect of Gold Nanoparticle Aggregation on Cell Uptake and Toxicity
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DOI：
10.1021/nn2007496
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发表时间：
2011-07-01
期刊：
影响因子：
17.1
通讯作者：
Chan, Warren C. W.
中科院分区：
文献类型：
--
作者：
Albanese, Alexandre;Chan, Warren C. W.
聚集似乎是所有纳米颗粒中普遍存在的现象，其在介导细胞摄取和相互作用中的影响尚不清楚。在这里，我们开发了一种简单的技术来生产不同大小的转铁蛋白包被的金纳米颗粒聚集体，并在三种不同的细胞系中表征其吸收和毒性。虽然聚集没有引起独特的毒性反应，但单个和聚集的纳米颗粒之间的摄取模式是不同的。与单个和单分散纳米颗粒相比，HeLa和A549细胞对聚集纳米颗粒的摄取减少了25%。然而，对于最大的合成聚集体，MDA-MB 435细胞摄取增加了2倍。这些对比的结果表明，细胞类型和相互作用的机制可能起着重要的作用。这项研究强调了需要在个案基础上研究细胞聚集体的行为，以及聚集体在介导靶向和细胞内运输中的重要性。
Aggregation appears to be a ubiquitous phenomenon among all nanoparticles and Its influence In mediating cellular uptake and Interactions remain unclear. Here we developed a simple technique to produce transferrin-coated gold nanoparticle aggregates of different sizes and characterized their uptake and toxicity In three different cell lines. While the aggregation did not elicit a unique toxic response, the uptake patterns were different between single and aggregated nanoparticles. There was a 25% decrease in uptake of aggregated nanoparticles with HeLa and A549 cells In comparison to single and monodisperse nanoparticles. However, there was a 2-fold increase in MDA-MB 435 cell uptake for the largest synthesized aggregates. These contrasting results suggest that cell type and the mechanism of interactions may play a significant role. This study highlights the need to investigate the behavior of aggregates with cells on a case-by-case basis and the importance of aggregation in mediating targeting and intracellular trafficking.