The Role of BCG/PPD‐Activated Macrophages in Resistance against Systemic Candidiasis in Mice
The Role of BCG/PPD‐Activated Macrophages in Resistance against Systemic Candidiasis in Mice
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DOI：
10.1111/j.1365-3083.1992.tb03132.x
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发表时间：
1992-11
影响因子：
3.7
通讯作者：
J. Wout;R. Poell;R. Furth
中科院分区：
文献类型：
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作者：
J. Wout;R. Poell;R. Furth
本研究的主要结论是，与对照小鼠的巨噬细胞相比，BCG/PPD活化的巨噬细胞表现出PMA诱导的H2 O2产生增加。杀死约三分之一的被吞噬的白色念珠菌，并对白色念珠菌芽管的细胞内形成造成50%以上的抑制。来自出生后用C.白念珠菌在PMA刺激下没有表现出H2 O2产生的增加，并且芽管的细胞内生长仅在有限程度上受到抑制。这些巨噬细胞能够杀死约20%摄入的白色念珠菌。在体内，静脉注射白色念珠菌后，BCG治疗小鼠中肾脏、脾脏和肝脏中的念珠菌数量显著低于对照小鼠。纳塔尔后C.白色念珠菌对静脉注射的白色念珠菌在脾脏和肝脏中的生长只有有限的影响，但不影响肾脏中的生长。这些结果表明，对系统性念珠菌感染的获得性免疫涉及细胞内杀伤的氧化和非氧化机制，这些机制可能对酵母和菌丝形式的念珠菌有不同的影响。白色念珠菌
The main conclusions of this study are that BCG/PPD‐activated macrophages, in contrast to macrophages from control mice, exhibit an increased PMA‐induced production of H2O2. kill about one‐third of the phagocytosed Candida albicans, and cause more than 5O% inhibition of the intracellular formation of germ tubes by C albicans. Peritoneal macrophages from mice that were colonized post‐natally with C. albicans do not show increased produciion of H2O2: upon stimulation with PMA and the intracellular outgrowth of germ tubes is inhibited to only a limited degree. These macrophages are capable of killing about 2O% of the ingested C, albicans. In vivo, the number of Candida in the kidney, spleen and liver after intravenous injection of Candida albicans is significantly lower in BCG‐treated mice than in control mice. Post‐natal colonization with C. albicans has only a limited effect on the outgrowth of intravenously injected C albicans the spleen and liver but does not influence growth in the kidney. These results indicate that acquired immunity against a systemic Candida infection involves both oxidative and nonoxidative mechanisms of intracellular killing and that these mechanisms may have different effects on the yeast and hyphal forms of C. albicans.