Thermus sediminis sp. nov., a thiosulfate-oxidizing and arsenate-reducing organism isolated from Little Hot Creek in the Long Valley Caldera, California
Thermus sediminis sp. nov., a thiosulfate-oxidizing and arsenate-reducing organism isolated from Little Hot Creek in the Long Valley Caldera, California
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栖热菌 sp.
DOI：
10.1007/s00792-018-1055-2
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发表时间：
2018
期刊：
影响因子：
2.9
通讯作者：
Brian P. Hedlund
中科院分区：
文献类型：
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作者：
En-Min Zhou;Chrisabelle C. Mefferd;Wen-Dong Xian;Scott C. Thomas;Arinola L. Adegboruwa;Nathan Williams;Senthil K. Murugapiran;Jeremy A. Dodsworth;Rakesh Ganji;Meng-Meng Li;Yi-Ping Ding;Lan Liu;Tanja Woyke;Wen-Jun Li;Brian P. Hedlund
热动物在自然和人工热环境中分布广泛。从美国加利福尼亚州东部的小热溪地热温泉中分离到两株新的黄色素菌株L198T和L423，被鉴定为属于热菌属的新生物。细胞呈革兰氏阴性，杆状，不活动。在45 ~ 75℃的温度和0 ~ 2.0%的NaCl添加浓度下观察到生长。这两种菌株通过硫代硫酸盐氧化成硫酸盐进行异养或趋化养生长。L198T和L423以砷酸盐为终端电子受体，通过有氧呼吸或厌氧呼吸生长。16S rRNA基因同源性（≤97.01%）、数字DNA-DNA杂交（≤32.7%）、OrthoANI（≤87.5%）和基因组-基因组距离（0.13）值均小于微生物种的既定标准。主要呼吸醌为甲基萘醌-8，主要细胞脂肪酸为iso-C15:0、iso-C17:0和前iso-C15:0。一种未识别的磷脂（PL1）和一种未识别的糖脂（GL1）主导了极性脂质模式。通过β-半乳糖苷酶和β-葡萄糖苷酶活性以及羟基脂肪酸的存在，可以将新菌株与相关分类群区分开来。基于系统发育、基因组学、表型和化学分类证据，提出了新种sediminis sp. nov.，类型菌株为L198T （= CGMCC 1.13590T = KCTC XXX）。
Thermus species are widespread in natural and artificial thermal environments. Two new yellow-pigmented strains, L198T and L423, isolated from Little Hot Creek, a geothermal spring in eastern California, were identified as novel organisms belonging to the genus Thermus. Cells are Gram-negative, rod-shaped, and non-motile. Growth was observed at temperatures from 45 to 75 °C and at salinities of 0–2.0% added NaCl. Both strains grow heterotrophically or chemolithotrophically by oxidation of thiosulfate to sulfate. L198T and L423 grow by aerobic respiration or anaerobic respiration with arsenate as the terminal electron acceptor. Values for 16S rRNA gene identity (≤ 97.01%), digital DNA–DNA hybridization (≤ 32.7%), OrthoANI (≤ 87.5%), and genome-to-genome distance (0.13) values to all Thermus genomes were less than established criteria for microbial species. The predominant respiratory quinone was menaquinone-8 and the major cellular fatty acids were iso-C15:0, iso-C17:0 and anteiso-C15:0. One unidentified phospholipid (PL1) and one unidentified glycolipid (GL1) dominated the polar lipid pattern. The new strains could be differentiated from related taxa by β-galactosidase and β-glucosidase activity and the presence of hydroxy fatty acids. Based on phylogenetic, genomic, phenotypic, and chemotaxonomic evidence, the novel species Thermus sediminis sp. nov. is proposed, with the type strain L198T (= CGMCC 1.13590T = KCTC XXX).