Effects of maternal undernutrition on the growth, development and antioxidant status of ovine placentome subtypes during late pregnancy
Effects of maternal undernutrition on the growth, development and antioxidant status of ovine placentome subtypes during late pregnancy
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孕晚期母体营养不良对绵羊胎盘亚型生长发育和抗氧化状态的影响
DOI：
10.1016/j.theriogenology.2018.01.002
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发表时间：
2018-04-01
期刊：
影响因子：
2.8
通讯作者：
Gao, Feng
中科院分区：
文献类型：
--
作者：
Liu, Yingchun;Li, Hui;Gao, Feng
本研究旨在探讨妊娠后期母体营养不良对绵羊胎盘组亚型生长发育及抗氧化状态的影响。在妊娠第90天将18只单胎蒙古母羊分为3组：限制组1 (RG1, 0.18 MJ ME)；kg BW-0.75 d(-1), n = 6)，限制组2 (RG2, 0.33 MJ ME。kg BW-035 d(-1), n = 6)和对照组（CG，任意，0.67 MJ ME）。公斤bw - 0.75。D (-1), n = 6)在限制期内给所有动物喂干草。妊娠140天切除妊娠子宫；然后对单个胎儿和胎盘进行称重。检测胎盘组亚型的数量和重量。苏木精-伊红染色切片，观察胎盘组各亚型的血管数量。采用分光光度法测定抗氧化能力和氧化应激指标，包括总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性和丙二醛（MDA）浓度。两组胎儿体重、胎盘重量和A型胎盘细胞数量均显著降低(P
This study investigated the effects of maternal undernutrition on the growth, development and antioxidant status of ovine placentome subtypes during late pregnancy. Eighteen time-mated Mongolian ewes with singleton fetuses were allocated to three groups at d 90 of pregnancy: Restricted Group 1 (RG1, 0.18 MJ ME.kg BW-0.75 d(-1), n = 6), Restricted Group 2 (RG2, 0.33 MJ ME.kg BW-035 d(-1), n = 6) and Control Group (CG, ad libitum, 0.67 MJ ME.kg BW-0.75.d(-1), n = 6). All animals were supplied chopped hay during the restriction period. At 140 days of pregnancy, the gravid uterus was removed; then individual fetuses and placentae were weighed. The numbers and weight of the placentome subtypes were examined. The number of vessels of the placentome subtypes were observed in sections stained with hematoxylin-eosin. The indexes of antioxidant capacity and oxidative stress, including total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity and mal-ondialdehyde (MDA) concentration, were measured by spectrometric kits. The fetal weights, placental weights and the numbers of Type A placentomes in both restricted groups were reduced (P