Effects of weight loss and exercise on the distribution of lead and essential trace elements in rats with prior lead exposure
Effects of weight loss and exercise on the distribution of lead and essential trace elements in rats with prior lead exposure
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DOI：
10.2307/3434458
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发表时间：
1999-08-01
影响因子：
10.4
通讯作者：
Bogden, JD
中科院分区：
文献类型：
--
作者：
Han, SG;Li, WJ;Bogden, JD
我们研究了体重减轻和非负重运动（游泳）对铅暴露大鼠血液和器官铅和必需金属浓度的影响。9周龄的雌性Sprague-Dawley大鼠（n = 37）在饮用水中加入醋酸铅2周，然后在4天的潜伏期内不接触铅。然后将大鼠随机分配至6个处理组之一：自由进食的体重维持、20%食物限制的中度体重减轻和40%食物限制的显著体重减轻，游泳或不游泳。每周测量血铅浓度。在4周的食物限制期后对大鼠实施安乐死，并收获脑、肝、肾、四头肌、腰椎骨和股骨，用于使用原子吸收分光光度法分析铅、钙、铜、铁、镁和锌。游泳和非游泳大鼠喂养限制饮食一贯较高的血铅浓度比自由控制。体重大幅下降组大鼠的器官铅浓度高于体重维持组大鼠。中度体重减轻组的大鼠具有中间值。游泳组和非游泳组之间的血液和器官铅浓度没有显着差异。器官铁浓度随体重减轻而增加，但研究的其他金属则没有。体重减轻也增加了非游泳大鼠的血细胞比容和骨密度降低。铅商店对体重减轻的反应类似于铁商店，因为两者都是在食物限制期间保存的，而不是研究的其他金属的商店减少。体重减轻，特别是快速体重减轻，可能会导致有铅过量暴露史的人出现铅中毒。
We studied the effects of weight loss and non-weight-bearing exercise (swimming) on blood and organ lead and essential metal concentrations in rats with prior lead exposure. Nine-week-old female Sprague-Dawley rats (n = 37) received lead acetate in their drinking water for 2 weeks, followed by a 4-day latency period without lead exposure. Rats were then randomly assigned to one of six treatment groups: weight maintenance with ad libitum feeding, moderate weight loss with 20% food restriction, and substantial weight loss with 40% food restriction, either with or without swimming. Blood lead concentrations were measured weekly. The rats were euthanized after a 4-week period of food restriction, and the brain, liver, kidneys, quadriceps muscle, lumbar spinal column bones, and femur were harvested for analysis for lead, calcium, copper, iron, magnesium, and zinc using atomic absorption spectrophotometry. Both swimming and nonswimming rats fed restricted diets had consistently higher blood lead concentrations than the ad libitum controls. Rats in the substantial weight loss group had higher organ lead concentrations than rats in the weight maintenance group. Rats in the moderate weight loss group had intermediate values. There were no significant differences in blood and organ lead concentrations between the swimming and nonswimming groups. Organ iron concentrations increased with weight loss, but those of the other metals studied did not. Weight loss also increased hematocrits and decreased bone density of the nonswimming rats. The response of lead stores to weight loss was similar to that of iron stores because both were conserved during food restriction in contrast to decreased stores of the other metals studied. It is possible that weight loss, especially rapid weight loss, could result in lead toxicity in people with a history of prior excessive lead exposure.