Tissular immunoenzymatic detection of hepatic alphafetoprotein in human hepatomas.
Tissular immunoenzymatic detection of hepatic alphafetoprotein in human hepatomas.
复制标题
人肝癌中肝甲胎蛋白的组织免疫酶检测。
作者：
S. Peyrol;J. Grimaud;J. Chayvialle;B. Veyre;P. Paliard;R. Lambert
本文选择了5例肝癌患者，根据其血清甲胎蛋白（alpha-fetoprotein，alpha-FP）水平和组织学特点进行比较。已经用特异性辣根过氧化物酶标记的免疫球蛋白研究了产生α-FP的细胞。超微结构检查显示，α-FP存在于一些肿瘤肝细胞的细胞质中。α-FP也存在于一些罕见的非分泌性肝癌的非肿瘤肝细胞的细胞质中。根据肿瘤细胞群的分化程度，描述了肿瘤肝细胞的超微结构差异。α-FP的产生似乎仅限于中度分化的肿瘤肝细胞。这些观察结果导致了这样的假设，即α-FP的产生可能在肿瘤肝细胞的分化过程中或在参与增殖过程的非肿瘤肝细胞的新分化过程中短暂地发展。
5 human cases of hepatoma have been chosen with respect to their different seric alpha-fetoprotein (alpha-FP) level and histological characters. Cells producing alpha-FP have been studied with specific horseradish-peroxidase-labelled immunoglobulins. Ultrastructural examination shows that alpha-FP is present in the cytoplasm of some tumoral hepatocytes. alpha-FP is also present in the cytoplasm of some rare nontumoral hepatocytes of a nonsecreting hepatoma. Ultrastructural differences are described in tumoral hepatocytes according to the grade of differentiation of the tumoral cell population. alpha-FP production appears to be restricted to moderately differentiated tumoral hepatocytes. These observations led to the hypothesis that production of alpha-FP may transiently develop either during the differentiation of tumoral hepatocytes, or during the new differentiation of nontumoral hepatocytes involved in a proliferative process.