Orientation-selected 15N-HYSCORE detection of weakly coupled nitrogens around the archaeal Rieske [2Fe-2S] center
Orientation-selected 15N-HYSCORE detection of weakly coupled nitrogens around the archaeal Rieske [2Fe-2S] center
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DOI：
10.1021/ja045898x
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发表时间：
2004-11-03
影响因子：
15
通讯作者：
Dikanov, SA
中科院分区：
文献类型：
--
作者：
Iwasaki, T;Kounosu, A;Dikanov, SA
均匀 15 N 标记的超热稳定古菌 Rieske 蛋白的还原 Rieske [2Fe−2S] 簇周围的弱耦合 15 N 原子似乎在 HYSCORE 光谱中产生易于观察到的交叉峰，除了协调的 Nδ 原子的贡献外，Nε 的耦合为 0.3−0.4 MHz，肽主链氮的耦合为 1.1 MHz。这些特征可用于涉及电子-质子耦合转移反应中弱耦合氮的生物氧化还原蛋白系统的结构机制研究。
The weakly coupled15N atoms around a reduced Rieske [2Fe−2S] cluster of the uniformly15N-labeled, hyperthermostable archaeal Rieske protein appear to produce readily observable cross-peaks in the HYSCORE spectra, with the well-resolved couplings of 0.3−0.4 MHz for the Nεand 1.1 MHz for the peptide backbone nitrogens, in addition to the contributions from the coordinated Nδatoms. These features can be used for structure-mechanism studies of the biological redox protein system involving the weakly coupled nitrogens in coupled electron−proton transfer reactions.