ACTIN-BASED BACTERIAL MOTILITY
ACTIN-BASED BACTERIAL MOTILITY
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DOI：
10.1016/0955-0674(95)80050-6
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发表时间：
1995-02-01
影响因子：
7.5
通讯作者：
COSSART, P
中科院分区：
文献类型：
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作者：
COSSART, P
单核细胞增生李斯特菌和其他细菌病原体在宿主细胞的细胞质中移动，由细菌一极的连续肌动蛋白组装推动。这种基于肌动蛋白的运动需要细菌蛋白ActA的存在。单核细胞增多症和。福氏志贺氏菌的IcsA。在过去的一年中有几个重大发现：发现ActA和IcsA在细菌表面的极性分布;证明细菌ActA在感染的宿主细胞中被磷酸化;参与一些宿主细胞蛋白，特别是profilin;以及ActA在转染的真核细胞中表达的显著效果。一个无细胞系统，忠实地重建肌动蛋白为基础的运动L。单核细胞增多症有望有助于进一步剖析这一迷人的现象。
Listeria monocytogenes and other bacterial pathogens move in the host cell cytoplasm, propelled by continuous actin assembly at one pole of the bacterium. This actin-based motility requires the presence of the bacterial proteins ActA on L. monocytogenes and. IcsA on Shigella flexneri. There have been several major discoveries in the past year: the discovery of the polar distribution of ActA and IcsA on the bacterial surface; the demonstration that bacterial ActA is phosphorylated in infected host cells; the involvement of some host cell proteins, particularly profilin; and the dramatic effect of ActA expression in transfected eukaryotic cells. A cell-free system that reconstitutes faithfully the actin-based motility of L. monocytogenes promises to be instrumental in the further dissection of this fascinating phenomenon.