MOLECULAR CHARACTERIZATION AND PHYLOGENETIC STUDIES OF A WOUND-INDUCIBLE PROTEINASE INHIBITOR-I GENE IN LYCOPERSICON SPECIES
MOLECULAR CHARACTERIZATION AND PHYLOGENETIC STUDIES OF A WOUND-INDUCIBLE PROTEINASE INHIBITOR-I GENE IN LYCOPERSICON SPECIES
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DOI：
10.1073/pnas.83.19.7277
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发表时间：
1986-10-01
影响因子：
11.1
通讯作者：
RYAN, CA
中科院分区：
文献类型：
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作者：
LEE, JS;BROWN, WE;RYAN, CA
一种编码蛋白酶抑制剂I的基因，其表达在番茄叶（Lycopersicon esculentum L.）变种Bonny Best），从基因组文库中分离并表征。核苷酸序列显示，该基因是完整的，并编码抑制剂I cDNA的序列，该抑制剂I cDNA是以前从从番茄叶片的伤口诱导的mRNA制备的cDNA文库中分离的。该基因位于抑制剂II基因上游13.1个酶对（kbp）处。创伤诱导基因被分别位于开放阅读框的氨基酸17和47的密码子内的445和404 bp的两个间插序列中断。除了存在推定的调节序列TATAAA和CCACT之外，两个拷贝的不完全的同向重复序列也存在。在5“-侧翼区鉴定出100 bp长。创伤诱导抑制剂I基因的各种番茄物种的基因组内的系统发育比较显示，重复被发现在七个祖先种的番茄。
A gene coding for proteinase inhibitor I, whose expression is induced in tomato leaves (Lycopersicon esculentum L. var. Bonny Best) in response to wounding or insect attacks, was isolated from a genomic library and characterized. The nucleotide sequence revealed that the gene is complete and encodes the sequence of an inhibitor I cDNA that was previously isolated from a cDNA library prepared from wound-induced mRNA from tomato leaves. This gene is located 13.1 kilobase pairs (kbp) upstream from an inhibitor II gene. The wound-inducible gene is interrupted by two intervening sequences of 445 and 404 bp, situated within the codons of amino acids 17 and 47, respectively, of the open reading frame. In addition to the presence of putative regulatory sequences TATAAA and CCACT, two copies of an imperfect direct repeat .apprxeq. 100 bp long were identified in the 5''-flanking region. Phylogenetic comparisons of wound-inducible inhibitor I genes within the genomes of various Lycopersicon species revealed that the repeat is found in seven ancestral species of tomato.