Evaluation of biologic sources of variation of leukocyte counts and other hematologic quantities using very precise automated analyzers.
Evaluation of biologic sources of variation of leukocyte counts and other hematologic quantities using very precise automated analyzers.
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DOI：
10.1093/ajcp/69.1.48
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发表时间：
1978
影响因子：
3.5
通讯作者：
B. Statland;P. Winkel;S. Harris;M. J. Burdsall;A. Saunders
中科院分区：
文献类型：
--
作者：
B. Statland;P. Winkel;S. Harris;M. J. Burdsall;A. Saunders
作者测定了20名健康成年志愿者的白细胞类型、血小板计数和红细胞值（红细胞比容、血红蛋白）的平均每天（5天以上）、平均每周（5周以上每周）和平均每小时（800小时、1100小时、1400小时）的生理个体内浓度变化。白细胞总数和白细胞类型的浓度值在Hemalong-DTM自动白细胞差异计数仪上一式两份测定，以尽量减少分析差异。根据方差分析模型对数据集进行分析。以%变异系数表示的每小时和每星期的平均生理个体内变异包括：血红蛋白，2.5,2.2；红细胞压积，2.6,2.7；血小板，1.6,6.6；白细胞总数，9.4,15.7；中性粒细胞，12.9,26.0；淋巴细胞，10.3,13.2；单核细胞，18.6,19.3；嗜酸性粒细胞，19.9,26.9；嗜碱性粒细胞，7.4,15.0；未染色的大细胞，13.7,16.0；“高过氧化物酶”细胞，32.7,25.7。小时变化分为随机日变化、群体一致日变化和受试者特定的独特个体变化。受试者特异性的日变化对许多白细胞类型的总日变化贡献很大，在统计上也很显著——尤其是嗜酸性粒细胞，它占总日变化的一半以上。
The authors determined mean day-to-day (daily over five days), mean week-to-week (weekly over five weeks) and mean hour-to-hour (800 h, 1100 h, 1400 h) physiologic intra-individual variation of concentration values of leukocyte cell types, platelet count, and erythrocyte values (hematocrit, hemoglobin) for a group of 20 healthy adult volunteers. Total leukocyte counts and concentration values of leukocyte cell types were determined in duplicate on the Hemalong-DTM Automated Leukocyte Differential Cell Counter to minimize analytic variation. The data sets were analyzed according to an analysis of variance model. The mean physiologic hour-to-hour and week-to-week intra-individual variations in terms of % coefficient of variation included: hemoglobin, 2.5, 2.2; hematocrit, 2.6, 2.7; platelets, 1.6, 6.6; total leukocyte count, 9.4, 15.7; neutrophils, 12.9, 26.0; lymphocytes, 10.3, 13.2; monocytes, 18.6, 19.3; eosinophils, 19.9, 26.9; basophils, 7.4, 15.0; large unstained cells, 13.7, 16.0; "high-peroxidase" cells, 32.7, 25.7. Hour-to-hour variation was partitioned into random diurnal variation, group-consistent diurnal variation, and subject-specific unique individual variation. Subject-specific diurnal variation contributed greatly and statistically significantly to total diurnal variation for many of the leukocyte cell types--especially in the case of eosinophils, where it represented more than half of total diurnal variation.