Polysaccharides from tubers of Bletilla striata: Physicochemical characterization, formulation of buccoadhesive wafers and preliminary study on treating oral ulcer
Polysaccharides from tubers of Bletilla striata: Physicochemical characterization, formulation of buccoadhesive wafers and preliminary study on treating oral ulcer
复制标题
白芨块茎多糖的理化性质、颊贴片的配方及治疗口腔溃疡的初步研究
DOI：
10.1016/j.ijbiomac.2018.09.050
复制
发表时间：
2019-02-01
影响因子：
8.2
通讯作者：
Qu, Yan
中科院分区：
文献类型：
--
作者：
Liao, Zhencheng;Zeng, Rui;Qu, Yan
本研究旨在唤起人们对白芨多糖（Bletilla striata多糖，BSP）的双重作用的认识，白芨多糖是一种具有多种药理活性的生物相容性、可生物降解的天然生物材料。首先对BSP进行了提取和表征。结果表明，BSP主要由甘露糖和葡萄糖组成。BSP的主链主要由(1 - bbb4)-连接的β - d -甘露吡喃糖组成。然后，以BSP和甘油浓度为自变量，采用a3(2)因子设计优化冻干硅片治疗外伤性口腔溃疡的效果。以拉伸强度、粘接强度和膨胀指数作为响应变量。确定了以含有4% (w/v) BSP和7% （w/v）甘油的溶液制备硅片的最佳配方。晶圆片具有足够的机械强度和合适的表面ph值。DSC/FGA热图显示了三峰失重事件和分解模式。体内实验表明，BSP硅片在第7天显著促进溃疡愈合，而在第12天无统计学差异。综上所述，本研究提示BSP是一种理想的粘膜粘连剂生物材料，在促进口腔溃疡愈合方面具有重要作用。(C) 2018 Elsevier B.V.版权所有
The purpose of this research is to arouse people's cognition of the dual role of Bletilla striata polysaccharide (BSP)-a kind of biocompatible, biodegradable natural biological materials with myriad pharmacological activities. BSP was extracted and characterized firstly. The results indicated that BSP was composed of mannose and glucose. The backbone of BSP mainly consisted of (1 -> 4)-linked beta-D-mannopyranose. Afterwards, A 3(2) factorial design was employed for optimization of lyophilized wafers for the treatment of traumatic oral ulcer with concentrations of BSP and glycerin as independent variables. Tensile strength, mucoadhesive strength, and swelling index were measured as response variables. It was determined that the wafer prepared from a solution containing 4% (w/v) BSP and 7% (w/v) glycerin was the optimal formulation. The wafers exhibited sufficient mechanical strength and suitable surface pH. Morphology of the wafers was observed. DSC/FGA thermograms showed the presence of three peaks weight loss events and decomposition patterns. Moreover, in vivo examinations indicated that the BSP wafer significantly promoted ulcer healing at 7 days, while no statistically significant difference was observed on day 12. In conclusion, this study suggests that BSP is an ideal biomaterial for mucosal adhesion agents, playing a nontrivial role in promoting oral ulcer healing. (C) 2018 Elsevier B.V. All rights reserved.