Phototrophic Bacteria as Feed Supplement for Rearing Penaeus monodon Larvae
Phototrophic Bacteria as Feed Supplement for Rearing Penaeus monodon Larvae
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光养细菌作为饲养斑节对虾幼虫的饲料添加剂
DOI：
10.1111/j.1749-7345.2002.tb00490.x
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
S. Vikineswary
中科院分区：
文献类型：
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作者：
Sujjat Al Azad;V. Chong;S. Vikineswary
基于以废水中培养的光养细菌硫化红螺菌（Rhodovulum sulfidophilum）、海洋硅藻中肋骨条藻（Skeletonema costatum）以及卤虫（Artemia）为食物，将斑节对虾（Penaeus monodon）的幼体从无节幼体阶段饲养到仔虾后期阶段。实验设计包括给溞状幼体投喂硅藻以及不同比例的细菌生物量作为食物，分别为D0（0%）、D1（1%）、D2（2%）、D3（3%）和D5（5%），而糠虾幼体和仔虾则投喂上述食物并补充卤虫无节幼体。对照组不投喂细菌生物量（食物D0）。在食物中添加硫化红螺菌生物量对斑节对虾幼体的生长和存活有显著影响。在第一个实验中，投喂食物D1（中肋骨条藻 + 1%硫化红螺菌）的幼体在投喂的第九天达到第一期仔虾阶段（PL1），平均总长度（TL）为6.13 ± 0.05毫米，这显著高于（P < 0.05）投喂食物D0的幼体（平均总长度为5.48 ± 0.04毫米）。在第二个实验中，投喂食物D2（中肋骨条藻 + 2%硫化红螺菌）的幼体在投喂的第十天达到PL1阶段，平均总长度为6.28 ± 0.06毫米，这显著高于（P < 0.05）投喂食物D0的幼体（平均总长度为5.62 ± 0.05毫米）。然而，在两个实验中，投喂不同食物的对虾幼体在各阶段的体长都有显著差异（P > 0.05），由含3%和5%细菌生物量的食物所获得的生长和存活率最低，这些食物也使养殖水体中的氨氮含量最高。添加超过2%的硫化红螺菌生物量显然会使养殖水体质量变差。
Larvae of the giant tiger prawn Penaeus monodon were reared from the naupliar to pos-tlarval stage, based on a diet of waste-grown phototrophic bacterium Rhodovulum sulfido-philum, the marine diatom Skeletonema costatum, and brine shrimp Artemia The experimental design consisted of feeding the protozoea with diatoms and varying proportions of bacterial biomass as diet D0 (0%), D1 (1%), D2 (2%), D3 (3%), and D5 (5%), while mysis and postlarvae were fed the above diet supplemented with Artemia nauplii. Controls were not fed with bacteria biomass (diet D0). The addition of R. sulfidophilum biomass in the diet had significant effects on the growth and survival of P. monodon larvae. In the first experiment, the larvae fed with diet D1 (Skeletonema+ 1%R. sulfidophilum) reached the first postlarval stage (PL1) on the ninth day of feeding and had a mean total length (TL) of 6.13 ± 0.05 mm, which was significantly higher (P 0.05). However, in both experiments prawn larvae fed with the various diets showed significant differences in their length-at-stage (P > 0.05), and the lowest growth and survival were obtained from diets comprising 3% and 5% bacterial biomass, which also gave the highest levels of ammonia-nitrogen in the culture water. Addition of more than 2% R. sulfidophilum biomass apparently deteriorates the quality of the culture water.