IMPORTANCE OF ENHANCED MASS RESOLUTION IN REMOVING INTERFERENCES WHEN MEASURING VOLATILE ORGANIC-COMPOUNDS IN HUMAN BLOOD BY USING PURGE-AND-TRAP GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
IMPORTANCE OF ENHANCED MASS RESOLUTION IN REMOVING INTERFERENCES WHEN MEASURING VOLATILE ORGANIC-COMPOUNDS IN HUMAN BLOOD BY USING PURGE-AND-TRAP GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
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DOI：
10.1016/1044-0305(92)80006-7
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发表时间：
1992-11-01
影响因子：
3.2
通讯作者：
PATTERSON, DG
中科院分区：
文献类型：
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作者：
BONIN, MA;ASHLEY, DL;PATTERSON, DG
可以从人体血液中清除的挥发性有机化合物（VOC）的数量如此之大，以至于无法通过毛细管气相色谱法完全分离。因此，用于通过质谱法定量目标化合物的单质量色谱图在四极质谱仪的标称（整数）质量分辨率下具有许多干扰。这些干扰的结果范围从定量的小误差到目标VOC的完全错误的结果。通过使用磁扇形质谱仪，可以通过降低感兴趣质量周围的离子色谱窗口以更高的分辨率去除标称质量下的这些干扰。在3000分辨率（10%谷值定义）下，可以为目标VOC的定量离子制作独特的单离子色谱图。需要全扫描质量数据来识别从血液中清除的未知化合物。通过使用同位素稀释质谱法，大多数目标VOC可以在低万亿分之一的范围内检测到10毫升的血液中的VOC已被清除和陷阱的方法。
The number of volatile organic compounds (VOCs) that can be purged from human blood is so great that they cannot be separated completely by capillary gas chromatography. As a result, the single-mass chromatograms used for quantitating the target compounds by mass spectrometry have many interferences at nominal (integer) mass resolution of a quadrupole mass spectrometer. The results of these interferences range from small errors in quantitation to completely erroneous results for the target VOCs. By using a magnetic sector mass spectrometer, these interferences at nominal mass can be removed at higher resolution by lowering the ion chromatogram windows around the masses of interest. At 3000 resolution (10% valley definition), unique single-ion chromatograms can be made for the quantitation ions of the target VOCs. Full-scan mass data are required to allow the identification of unknown compounds purged from the blood. By using isotope-dilution mass spectrometry, most target VOCs can be detected in the low parts per trillion range for a 10-mL quantity of blood from which the VOCs have been removed by a purge-and-trap method.