Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometry
Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometry
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DOI：
10.1039/c4ra12021c
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发表时间：
2015-01-01
期刊：
影响因子：
3.9
通讯作者：
Watanabe, Tomonobu M.
中科院分区：
文献类型：
--
作者：
Fujita, Hideaki;Esaki, Tsuyoshi;Watanabe, Tomonobu M.
分泌组学，细胞释放的综合研究，提供了一个非侵入性的方法来了解细胞异质性。在这里，我们提出了单细胞小分子分泌组学使用微滴阵列和质谱作为一个新的组学技术的剧目。本发明的方法揭示了来自个体单细胞的分泌小分子的异质性，而不使用任何侵入性过程。
Secretomics, the comprehensive study of cell releasates, offers a non-invasive approach to understanding cell heterogeneity. We here propose single cell small molecule secretomics using micro-droplet arrays and mass spectrometry as a new repertoire of omics technologies. The present method revealed the heterogeneity of secreted small molecules from individual single cells without the use of any invasive processes.