Large-Scale Calcium Waves Traveling through Astrocytic Networks In Vivo
Large-Scale Calcium Waves Traveling through Astrocytic Networks In Vivo
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DOI：
10.1523/jneurosci.5319-10.2011
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发表时间：
2011-02-16
影响因子：
5.3
通讯作者：
Ikegaya, Yuji
中科院分区：
文献类型：
--
作者：
Kuga, Nahoko;Sasaki, Takuya;Ikegaya, Yuji
脑血流的宏观变化，如脑功能成像所捕获的变化，需要与神经元和脑血管网络相互作用的星形胶质细胞、神经胶质细胞的高度组织化、大规模动态。然而，星形胶质细胞的活动主要是在单个细胞的水平上进行研究，缺乏关于它们集体行为的信息。在这项工作中，我们在体内同时监测了数百只小鼠海马星形胶质细胞的钙活性，发现几乎所有的星形胶质细胞都参与了从细胞到细胞传播的再生波（这里称为“glissandi”）。Glissandi出现依赖于神经元的活动，并伴随着降低局部场电位的次声波动和减少红细胞的流动。这一新的现象在此之前被忽视了，可能是因为星形胶质细胞对光损伤的高度脆弱性; glissandi只有在比以前使用的低得多的激光强度下观察时才会发生。
Macroscopic changes in cerebral blood flow, such as those captured by functional imaging of the brain, require highly organized, large-scale dynamics of astrocytes, glial cells that interact with both neuronal and cerebrovascular networks. However, astrocyte activity has been studied mainly at the level of individual cells, and information regarding their collective behavior is lacking. In this work, we monitored calcium activity simultaneously from hundreds of mouse hippocampal astrocytes in vivo and found that almost all astrocytes participated en masse in regenerative waves that propagated from cell to cell (referred to here as "glissandi"). Glissandi emerged depending on the neuronal activity and accompanied a reduction in infraslow fluctuations of local field potentials and a decrease in the flow of red blood cells. This novel phenomenon was heretofore overlooked, probably because of the high vulnerability of astrocytes to light damage; glissandi occurred only when observed at much lower laser intensities than previously used.