New look inside the spindle: microtubule-dependent microtubule generation within the spindle
New look inside the spindle: microtubule-dependent microtubule generation within the spindle
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DOI：
10.1016/j.ceb.2009.11.012
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发表时间：
2010-02-01
影响因子：
7.5
通讯作者：
Kimura, Akatsuki
中科院分区：
文献类型：
--
作者：
Goshima, Gohta;Kimura, Akatsuki
通过显微技术的进步、相关基因的鉴定以及理论框架的构建，有丝分裂和减数分裂纺锤体的结构、动力学和机制已被逐步阐明。在这里，我们回顾了最近利用定量图像分析来加深我们对动物细胞复杂纺锤体结构的理解的工作。我们特别讨论了微管（MT）如何在纺锤体内部成核和分布。越来越多的证据支持纺锤体内存在依赖于 MT 的 MT 生成。这种机制将产生不同长度的纺锤内 MT 的密集阵列，这可能有助于有效的纺锤体组装并稳定中期纺锤体。通过定量图像分析进行 RNA 干扰 (RNAi) 筛选，鉴定出了在 MT 生成过程中发挥关键作用的 augmin 复合物。
The structure, dynamics, and mechanics of mitotic and meiotic spindles have been progressively elucidated through the advancements in microscopic technology, identification of the genes involved, and construction of theoretical frameworks. Here, we review recent works that have utilized quantitative image analysis to advance our understanding of the complex spindle structure of animal cells. In particular, we discuss how microtubules (MTs) are nucleated and distributed inside the spindle. Accumulating evidence supports the presence of MT-dependent MT generation within the spindle. This mechanism would produce dense arrays of intraspindle MTs with various lengths, which may contribute to efficient spindle assembly and stabilize the metaphase spindle. RNA interference (RNAi) screens with quantitative image analysis led to the identification of the augmin complex that plays a key role in this MT generation process.