Neuroprotective effect of the traditional Chinese herbal formula Tongxinluo: a PET imaging study in rats
Neuroprotective effect of the traditional Chinese herbal formula Tongxinluo: a PET imaging study in rats
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中药通心络的神经保护作用：大鼠 PET 成像研究
DOI：
10.4103/1673-5374.137573
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发表时间：
2014-07-01
影响因子：
6.1
通讯作者：
Cai, Yefeng
中科院分区：
文献类型：
--
作者：
Cheng, Xiao;Luo, Haoxuan;Cai, Yefeng
通心络在中国治疗中风急性期和神经保护方面应用广泛。然而，关于通心络对小动物脑缺血/再灌注损伤的保护作用的正电子发射断层扫描(PET)研究较少。本实验采用大鼠大脑中动脉闭塞前连续5天灌胃通心络超微粉混悬液或其载药剂。18F-脱氧葡萄糖(FDG)小动物PET显像显示，脑缺血再灌流后1周和2周，通心络治疗组大鼠缺血区葡萄糖代谢明显高于赋形剂组。尼氏染色显示，脑缺血再灌流2周后，通心络治疗组大鼠前额叶皮质神经元丢失明显少于对照组。此外，与接受赋形剂的大鼠相比，通心络治疗的动物表现出更好的神经功能和更小的脑梗塞体积。提示通心络对脑缺血再灌注损伤具有神经保护作用，18F-FDG小动物PET显像是研究中药分子药理的有效工具。
Tongxinluo has been widely used in China for the treatment of acute stroke and for neuroprotection. However, there are few positron emission tomography (PET) studies on the neuroprotective effect of Tongxinluo on cerebral ischemia/reperfusion in small animals. In the present study, Tongxinluo superfine powder suspension or its vehicle was administered intragastrically to rats for 5 successive days before middle cerebral artery occlusion. 18F-fluorodeoxyglucose (FDG) small animal PET imaging showed that at 1 and 2 weeks after cerebral ischemia/reperfusion, glucose metabolism in the ischemic area was greater in rats that had received Tongxinluo than in those that had received the vehicle. Nissl staining showed that 2 weeks after cerebral ischemia/reperfusion, there was less neuronal loss in the prefrontal cortex in Tongxinluo-treated rats than in controls. In addition, Tongxinluo-treated animals showed better neurologic function and lower cerebral infarct volume than rats that received the vehicle. These findings suggest that Tongxinluo exhibits neuroprotective effects in cerebral ischemia/reperfusion injury and demonstrates that 18F-FDG small animal PET imaging is a useful tool with which to study the molecular pharmacology of traditional Chinese medicine.