A DISTINCT AFRICAN LENTIVIRUS FROM SYKES MONKEYS
A DISTINCT AFRICAN LENTIVIRUS FROM SYKES MONKEYS
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DOI：
10.1128/jvi.67.3.1517-1528.1993
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发表时间：
1993-03-01
影响因子：
5.4
通讯作者：
JOHNSON, PR
中科院分区：
文献类型：
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作者：
HIRSCH, VM;DAPOLITO, GA;JOHNSON, PR
非洲赛克斯猴 (Cercopithecus mitis albogularis) 已证实存在猿猴免疫缺陷病毒 (SIV) 无症状感染，病毒分离证实赛克斯猴 (SIVsyk) 感染了一种新型 SIV。接种 SIVsyk 的猕猴持续感染，但仍保持临床健康。我们利用聚合酶链式反应扩增来生成 SIVsyk 的全长感染性分子克隆。 SIVsyk 的基因组结构与其他灵长类慢病毒相似，由 gag、pol、vif、vpr、tat、rev、env 和 nef 组成。一个独特的特征是长末端重复中不存在高度保守的 NF-KB 结合位点。 SIVsyk 与其他灵长类慢病毒在基因上等距。因此，SIVsyk代表了一个新的病毒群，与之前公认的四种灵长类慢病毒群不同：人类免疫缺陷病毒1型（HIV-1）、来自乌白眉猴的SIV（SIVsmm）和HIV-2、来自非洲绿猴的SIV（SIVagm）和来自山魈的SIV（SIVmnd）。 SIVsyk 和 SIVagm（源自同一属猴的分离株）之间的遗传差异强调了其他尚未分离和表征的不同 SIV 的潜力。
Asymptomatic infection with simian immunodeficiency virus (SIV) has been demonstrated in African Sykes' monkeys (Cercopithecus mitis albogularis), and virus isolation confirmed infection with a novel SIV from Sykes' monkeys (SIVsyk). Macaques inoculated with SIVsyk became persistently infected but remained clinically healthy. We utilized polymerase chain reaction amplification to generate a full-length, infectious molecular clone of SIVsyk. The genome organization of SIVsyk is similar to that of the other primate lentiviruses, consisting of gag, pol, vif, vpr, tat, rev, env, and nef. A unique feature is the absence of the highly conserved NF-KB binding site in the long terminal repeat. SIVsyk is genetically equidistant from other primate lentiviruses. Thus, SIVsyk represents a new group that is distinct from the four previously recognized primate lentivirus groups: human immunodeficiency virus type 1 (HIV-1), SIV from sooty mangabeys (SIVsmm) and HIV-2, SIV from African green monkeys (SIVagm), and SIV from mandrills (SIVmnd). The genetic differences between SIVsyk and SIVagm, isolates derived from monkeys of the same genus, underscore the potential for other distinct SIVs which have yet to be isolated and characterized.