Hyperactive mutant of a wheat plasma membrane Na+/H+ antiporter improves the growth and salt tolerance of transgenic tobacco.
Hyperactive mutant of a wheat plasma membrane Na+/H+ antiporter improves the growth and salt tolerance of transgenic tobacco.
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DOI：
10.1016/j.plantsci.2016.09.016
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发表时间：
2016-12
期刊：
影响因子：
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通讯作者：
Yang Zhou;Zesen Lai;Xiaochang Yin;Shan Yu;Yuan-yuan Xu;Xiaoxiao Wang;Xinli Cong;Yuehua Luo
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文献类型：
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作者：
Yang Zhou;Zesen Lai;Xiaochang Yin;Shan Yu;Yuan-yuan Xu;Xiaoxiao Wang;Xinli Cong;Yuehua Luo
小麦SOS 1（TaSOS 1）的C-末端168个残基（自抑制结构域）的缺失可以减轻其活性。这种截短形式的小麦SOS 1（TaSOS 1 -974）被证明增加补偿（与野生型TaSOS 1相比）的盐敏感性的酵母突变株，AXT 3 K，通过增加Na+运输出细胞在盐度胁迫。与正常条件下的野生型植物相比，质膜蛋白TaSOS 1 -974或TaSOS 1的表达改善了转基因烟草植物的生长。然而，表达TaSOS 1 - 974的植物比TaSOS 1转化的植物生长更好。在盐胁迫下，转TaSOS 1 - 974和TaSOS 1基因植株根中Na+外流和K+内流速率均大于野生型植株。此外，与转TaSOS 1基因植株相比，TaSOS 1 -974基因表达植株的Na+外流和K+内流速度更快，导致转TaSOS 1 -974基因植株的Na+积累量低于转TaSOS 1基因植株和野生型植株，而K+积累量高于转TaSOS 1基因植株。表明TaSOS 1 -974可能保护质膜免受盐胁迫产生的氧化损伤。总体而言，与TaSOS 1相比，TaSOS 1 -974在转基因植物中赋予更高的耐盐性。与此结果一致，表达TaSOS 1 - 974的转基因植物在盐条件下表现出比表达TaSOS 1和野生型植物更好的生长性能。
Wheat SOS1 (TaSOS1) activity could be relieved upon deletion of the C-terminal 168 residues (the auto-inhibitory domain). This truncated form of wheat SOS1 (TaSOS1-974) was shown to increase compensation (compared to wild-type TaSOS1) for the salt sensitivity of a yeast mutant strain, AXT3K, via increased Na+transportation out of cells during salinity stress. Expression of the plasma membrane proteins TaSOS1-974 or TaSOS1 improved the growth of transgenic tobacco plants compared with wild-type plants under normal conditions. However, plants expressingTaSOS1-974grew better thanTaSOS1-transformed plants. Upon salinity stress, Na+efflux and K+influx rates in the roots of transgenic plants expressingTaSOS1-974orTaSOS1were greater than those of wild-type plants. Furthermore, compared toTaSOS1-transgenic plants,TaSOS1-974-expressing roots showed faster Na+efflux and K+influx, resulting in less Na+and more K+accumulation inTaSOS1-974-transgenic plants compared toTaSOS1-transgenic and wild-type plants.TaSOS1-974-expressing plants had the lowest MDA content and electrolyte leakage among all tested plants, indicating that TaSOS1-974 might protect the plasma membrane against oxidative damage generated by salt stress. Overall, TaSOS1-974 conferred higher salt tolerance in transgenic plants compared to TaSOS1. Consistent with this result, transgenic plants expressingTaSOS1-974showed a better growth performance thanTaSOS1-expressing and wild-type plants under saline conditions.