Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein–DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region
Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein–DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region
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涉及 AML1/Runx-1/CBFα Runt 结构域、CBFβ 和 C/EBPβ bZip 区域的四元、三元和二元蛋白质-DNA 复合物的结晶和初步 X 射线分析
DOI：
10.1107/s0907444901003900
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
K. Ogata
中科院分区：
文献类型：
--
作者：
T. Tahirov;T. Inoue;M. Sasaki;M. Shiina;K. Kimura;Ko Sato;T. Kumasaka;Masaki Yamamoto;N. Kamiya;K. Ogata
结果表明，AML 1/Runx-1/CBFα（Runt）-CBFβ-C/EBPβ（bZip）-DNA（CBFα-β-C/EBPβ-DNA）、AML 1/Runx-1/CBFα（Runt）-C/EBPβ（bZip）-DNA（CBFα-C/EBPβ-DNA）和AML 1/Runx-1/CBFα（Runt）-DNA（CBFα-DNA）三种蛋白质-DNA复合物结晶。晶体均为正交晶系，空间群分别为C2221、P21212和P212121。CBFα-β-C/EBPβ-DNA和CBFα-C/EBPβ-DNA晶体的分辨率均为3 A，而CBFα-DNA晶体的分辨率为2.65 A。 收集了所有天然晶体的完整数据集，沿着CBFα-β-C/EBPβ-DNA的MAD和MIR数据集。利用MAD数据确定了CBFα-β-C/EBPβ-DNA金衍生物的重原子位置。
Three types of protein–DNA complexes, AML1/Runx-1/CBFα(Runt)–CBFβ–C/EBPβ(bZip)–DNA (CBFα-β-C/EBPβ-DNA), AML1/Runx-1/CBFα(Runt)–C/EBPβ(bZip)–DNA (CBFα-C/EBPβ-DNA) and AML1/Runx-1/CBFα(Runt)–DNA (CBFα-DNA), were crystallized. The crystals were all orthorhombic and belonged to space groups C2221, P21212 and P212121, respectively. The resolutions of CBFα-β-C/EBPβ-DNA and CBFα-C/EBPβ-DNA crystals were both 3 A, while that of the CBFα-DNA crystal was 2.65 A. Complete data sets were collected for all of the native crystals, along with MAD and MIR data sets for CBFα-β-C/EBPβ-DNA. The heavy-atom site was determined using MAD data for a gold derivative of CBFα-β-C/EBPβ-DNA.