Sequence-specific molecular lithography on single DNA molecules
Sequence-specific molecular lithography on single DNA molecules
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DOI：
10.1126/science.1071247
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发表时间：
2002-07-05
期刊：
影响因子：
56.9
通讯作者：
Braun, E
中科院分区：
文献类型：
--
作者：
Keren, K;Krueger, M;Braun, E
在实现单个分子尺度电子器件方面的最新进展强调了对能够将此类器件组装成大规模功能电路的新型工具和概念的需求。我们通过利用RecA蛋白的同源重组，在底物DNA分子上展示了序列特异性分子光刻技术。我们以序列特异性的方式，对DNA的金属涂层进行了图案化，定位了标记的分子物体，并在DNA底物的特定位置上生长了金属岛，还生成了分子精度的稳定DNA连接点，用于对DNA底物的连接性进行图案化。在我们的分子光刻技术中，DNA分子中编码的信息取代了传统微电子学中使用的掩模，而RecA蛋白则充当了抗蚀剂。这种分子光刻技术在从纳米到许多微米的广泛长度尺度上都能以高分辨率工作。
Recent advances in the realization of individual molecular-scale electronic devices emphasize the need for novel tools and concepts capable of assembling such devices into large-scale functional circuits. We demonstrated sequence-specific molecular lithography on substrate DNA molecules by harnessing homologous recombination by RecA protein. In a sequence-specific manner, we patterned the coating of DNA with metal, localized labeled molecular objects and grew metal islands on specific sites along the DNA substrate, and generated molecularly accurate stable DNA junctions for patterning the DNA substrate connectivity. In our molecular lithography, the information encoded in the DNA molecules replaces the masks used in conventional microelectronics, and the RecA protein serves as the resist. The molecular lithography works with high resolution over a broad range of length scales from nanometers to many micrometers.