Transplantation of adult spinal cord grafts into spinal cord transected rats improves their locomotor function
Transplantation of adult spinal cord grafts into spinal cord transected rats improves their locomotor function
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DOI：
10.1007/s11427-019-9490-8
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发表时间：
2019-03
期刊：
影响因子：
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通讯作者：
He Shen;Xi Chen;Xing Li;Ke-Rong Jia;Zhifeng Xiao;Jianwu Dai
中科院分区：
文献类型：
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作者：
He Shen;Xi Chen;Xing Li;Ke-Rong Jia;Zhifeng Xiao;Jianwu Dai
胚胎中枢神经组织块移植可恢复新生大鼠脊髓半切后的功能，促进成年大鼠脊髓轴突生长。然而，成年人的脊髓节段还没有被用作同种异体移植的供体节段。在这里，我们利用成人脊髓组织移植物（aSCG）作为供体结构修复完全性脊髓损伤（SCI）。此外，为SCI治疗提供有利的微环境，将含有三种生长因子（脑源性神经营养因子、神经营养因子-3和血管内皮生长因子）的生长因子鸡尾酒应用于aSCG移植物。我们发现，在成年大鼠脊髓损伤部位移植aSCGs后12周，运动功能显著改善。aSCG与这些生长因子结合的移植增强了神经元和少突胶质细胞的存活和功能恢复。这些令人鼓舞的结果表明，通过移植aSCG治疗完全SCI，特别是在存在生长因子的情况下，对运动功能恢复具有积极作用，因此可以被认为是SCI的一种可能的治疗策略。
Grafted embryonic central neural tissue pieces can recover function of hemisected spinal cord in neonatal rats and promote axonal growth in adults. However, spinal cord segments from adults have not been used as donor segments for allogeneic transplantation. Here, we utilized adult spinal cord tissue grafts (aSCGs) as donor constructs for repairing complete spinal cord injury (SCI). Moreover, to provide a favourable microenvironment for SCI treatment, a growth factor cocktail containing three growth factors (brain-derived neurotrophic factor, neurotrophin-3 and vascular endothelial growth factor), was applied to the aSCG transplants. We found that the locomotor function was significantly improved 12 weeks after transplantation of aSCGs into the spinal cord lesion site in adult rats. Transplantation of aSCGs combined with these growth factors enhanced neuron and oligodendrocyte survival and functional restoration. These encouraging results indicate that treatment of complete SCI by transplanting aSCGs, especially in the presence of growth factors, has a positive effect on motor functional recovery, and therefore could be considered as a possible therapeutic strategy for SCI.