Estrogen induces CCN5 expression in the rat uterus in vivo
Estrogen induces CCN5 expression in the rat uterus in vivo
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DOI：
10.1210/en.2003-0823
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发表时间：
2004-02-01
期刊：
影响因子：
4.8
通讯作者：
Castellot, JJ
中科院分区：
文献类型：
--
作者：
Mason, HR;Grove-Strawser, D;Castellot, JJ
雌激素在子宫的正常生理和各种病理中起着重要作用。鉴于生殖周期和妊娠期间子宫重塑的性质，我们试图确定CCN5（我们已证明对平滑肌细胞增殖和迁移至关重要的基因）是否是子宫中雌激素诱导的基因。在本研究中，我们证明，发情前期雌性子宫中的 CCN5 mRNA 和蛋白表达水平比动情末期雌性高 5 倍，这一发现与雌激素对 CCN5 基因的诱导一致。用外源性雌激素或雌激素和孕酮治疗的切除卵巢的大鼠表现出比仅用孕酮或媒介物治疗的动物高4至8倍的CCN5 mRNA和蛋白质水平。使用免疫组织化学对大鼠子宫切片进行的分析表明，CCN5 定位于整个子宫，包括子宫内膜和子宫内膜腺体以及子宫肌层。因此，我们的数据表明CCN5在大鼠子宫中受到雌激素的正向调节，并表明该基因可能在维持正常子宫生理学中发挥重要作用。
Estrogen plays an important role in the normal physiology as well as various pathologies of the uterus. Given the nature of uterine remodeling during the reproductive cycle and pregnancy, we sought to determine whether CCN5, a gene that we have shown to be important in smooth muscle cell proliferation and migration, is an estrogen-induced gene in the uterus. In the present study, we demonstrate that levels of CCN5 mRNA and protein expression were 5-fold higher in uteri from proestrous females relative to metestrous females, a finding consistent with estrogen induction of the CCN5 gene. Ovariectomized rats treated with exogenous estrogen or estrogen and progesterone exhibited 4- to 8-fold higher levels of CCN5 mRNA and protein than animals treated with either progesterone or vehicle alone. Analysis of rat uterine sections using immunohistochemistry demonstrates CCN5 localization throughout the uterus, including the endometrium and endometrial glands as well as the myometrium. Thus, our data indicate that CCN5 is positively regulated by estrogen in the rat uterus and suggests that this gene may play an important role in maintaining normal uterine physiology.