REVERSIBLE ELECTRICAL BREAKDOWN OF LIPID BILAYERS - FORMATION AND EVOLUTION OF PORES
REVERSIBLE ELECTRICAL BREAKDOWN OF LIPID BILAYERS - FORMATION AND EVOLUTION OF PORES
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DOI：
10.1016/0005-2736(88)90202-7
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发表时间：
1988-05-24
期刊：
影响因子：
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通讯作者：
SOKIRKO, AI
中科院分区：
文献类型：
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作者：
GLASER, RW;LEIKIN, SL;SOKIRKO, AI
研究了脂质膜可逆电击穿的机理。孔隙发育过程的以下阶段得到证实。疏水孔是脂质双分子层自发波动形成的。如果这些充满水的缺陷扩展到0.3 ~ 0.5 nm的半径，则脂质分子的重定向形成亲水孔。细胞膜上的电位差有利于这一过程。孔的电导率取决于膜电压，这种依赖的类型随着孔的半径而变化。形成了有效半径为0.6 ~ 1nm的亲水性孔隙，这是观察到的膜电导率增加的原因。研究了电压脉冲期间和脉冲后平均半径和孔隙数变化的特征次数。
The mechanism of reversible electric breakdown of lipid membranes is studied. The following stages of the process of pore development are substantiated. Hydrophobic pores are formed in the lipid bilayer by spontaneous fluctuations. If these water-filled defects extend to a radius of 0.3 to 0.5 nm, a hydrophilic pore is formed by reorientation of the lipid molecules. This process is favoured by a potential difference across the mmbrane. The conductivity of the pores depends on membrane voltage, and the type of this dependence changes with the radius of the pore. Hydrophilic pores of an effective radius of 0.6 up to more than 1 nm are formed, which account for the membrane conductivity increase observed. The characteristic times of changes in average radius and number of pores during the voltage pulse and after it are investigated.