Molecular insights into neurodevelopmental and neurodegenerative diseases
Molecular insights into neurodevelopmental and neurodegenerative diseases
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DOI：
10.1016/s0361-9230(00)00376-2
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发表时间：
2000-11-01
影响因子：
3.8
通讯作者：
Stanley, JA
中科院分区：
文献类型：
--
作者：
Pettegrew, JW;Klunk, WE;Stanley, JA
磁共振波谱 (MRS) 是一种非侵入性物理技术，通常用于确定溶液中有机分子的数量和结构。扩大该技术用途的技术进步包括：(1) 高分辨率 MRS，用于识别和量化组织提取物的复杂混合物中存在的单个分子； (2) 体内 MRS 技术，用于非侵入性监测人体代谢物； (3)中等大小蛋白质的结构测定； （4）改进固体和液晶的结构表征，例如膜中相变的检测。本次审查的重点是上面提到的前两项技术进步。 MRS 作为研究疾病状态分子变化的研究工具，其优点包括易于样品制备、最少的样品操作、避免制备衍生物以及分析未分级样品的能力。 MRS 在临床上的优势在于它能够非侵入性地测量人体神经元代谢物水平，及其在疾病诊断、监测疾病进展和评估实验疗法疗效方面的潜力。 (C) 2000 爱思唯尔科学公司。
Magnetic resonance spectroscopy (MRS) is a noninvasive physical technique that is routinely used to determine the quantity and structure of organic molecules in solution, Technical advances that have expanded the usefulness of this technique include: (1) high resolution MRS to identify and quantify individual molecules present in complex mixtures of tissue extracts; (2) in vivo MRS techniques to non-invasively monitor metabolites in humans; (3) stucture determination of proteins of moderate size; and (4) improved structure characterization of solids and liquid crystals, such as the detection of phase changes in membranes. The focus of this review is on the first two technical advances mentioned above. The strengths of MRS as a research tool to investigate molecular alterations in disease states include ease of sample preparation, minimum sample manipulation, avoidance of the preparation of derivatives, and the ability to analyze an unfractionated sample. The strengths of MRS in the clinic are its ability to measure neuronal metabolite levels non-invasively in humans and its potential for disease diagnosis, monitoring disease progression, and assessing the efficacy of experimental therapies. (C) 2000 Elsevier Science Inc.