ION-RETARDATION DESALTING OF BLOOD AND OTHER ANIMAL-TISSUES FOR SEPARATION OF SOLUBLE METABOLITES BY 2-DIMENSIONAL CHROMATOGRAPHY
ION-RETARDATION DESALTING OF BLOOD AND OTHER ANIMAL-TISSUES FOR SEPARATION OF SOLUBLE METABOLITES BY 2-DIMENSIONAL CHROMATOGRAPHY
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DOI：
10.1016/0003-2697(77)90061-6
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发表时间：
1977-01-01
影响因子：
2.9
通讯作者：
BASSHAM, JA
中科院分区：
文献类型：
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作者：
BROOKS, GA;BISSELL, MJ;BASSHAM, JA
哺乳动物组织[人血、大鼠血、肝脏、肌肉]的酸性提取物中的代谢物通过AG11A8离子阻挡树脂进行脱盐。丙氨酸、天冬氨酸、葡萄糖、谷氨酸、谷氨酰胺、乳酸、亮氨酸和麦芽糖的定量回收率分别为95%、100%、92%、85%、96%、90%、97%和100%。有效的脱盐使组织提取物中存在的代谢物可以通过二维纸色谱分离。
Metabolites present in acid extracts of mammalian tissues [human blood, rat blood, liver, muscle] were desalted by passing the extracts through AG11A8 ion-retardation resin. Quantitative recoveries of alanine, aspartate, glucose, glutamate, glutamine, lactate, leucine and maltose were 95, 100, 92, 85, 96, 90, 97 and 100%, respectively. Effective desalting allows metabolites present in tissue extracts to be separated by 2-dimensional paper chromatography.