Effects of dietary β-1,3-glucan addition on the growth performance, mRNA expression in jejunal barrier, and cecal microflora of broilers challenged with Clostridium perfringens.
Effects of dietary β-1,3-glucan addition on the growth performance, mRNA expression in jejunal barrier, and cecal microflora of broilers challenged with Clostridium perfringens.
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DOI：
10.1016/j.psj.2022.102349
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发表时间：
2023-02
期刊：
影响因子：
4.4
通讯作者：
Xiaojun Yang
中科院分区：
文献类型：
--
作者：
Wei Guo;Di Han;Fei Zhang;Qinyi Zhan;Yanyan Liu;Qingyun Peng;Shengshu Huang;Zhen Xue;Xiaojun Yang
本试验旨在探讨β-1，3-葡聚糖和产气荚膜梭菌对肉仔鸡生长性能、肠道健康和盲肠菌群的影响。试验选用1日龄爱拔益加肉仔鸡384只，随机分为4个处理，6个重复。试验分为2个因素：日粮中β-1，3-葡聚糖添加量（0和250 mg/kg）、产气荚膜梭菌攻击或未攻击肠道肠炎。结果表明：21日龄时，产气荚膜梭菌感染通过降低空肠Occludin和Claudin-1 mRNA的表达，破坏了肠黏膜的完整性（P < 0.05）。同时，以产气荚膜梭菌为主要作用时，21 d葡萄糖转运蛋白重组钠/葡萄糖协同转运蛋白1（SGLT 1）和42 d脂肪酸转运蛋白肝脂肪酸结合蛋白（L-FABP）mRNA的表达也明显降低（P <0. 05），盲肠微生物多样性也受到影响，尤其是厚壁菌门和拟杆菌门的相对丰度。此外，产气荚膜梭菌感染使肉仔鸡42 d体重、日增重和料重比显著降低（P < 0.05）。日粮中添加β-1，3-葡聚糖可在一定程度上减轻产气荚膜梭菌引起的肠粘膜损伤。以β-1，3-葡聚糖为主时，42日龄时SGLT 1增加（P < 0.05），稳定了产气荚膜梭菌引起的肠道菌群失调。添加过量β-1，3-葡聚糖可显著提高42日龄日增重（P < 0.05），提高1 ~ 42日龄日增重和饲料转化率（P < 0.05）。总之，日粮中添加β-1，3-葡聚糖可以改善感染产气荚膜梭菌的肉鸡的生长性能和肠道健康。
This experiment aimed to explore the interaction of β-1,3-glucan and Clostridium perfringens on the growth performance, intestinal health and cecal microflora of broilers. A total of 384 one-day-old Arbor Acre broilers were sorted into 4 treatments with 6 replications. There were 2 factors in this trial: dietary β-1,3-glucan addition including 0 and 250 mg/kg, intestinal enteritis challenged with Clostridium perfringens attack or not. Results showed that Clostridium perfringens infection disrupted the integrity of the intestinal mucosa by reducing the jejunal Occludin and Claudin-1 mRNA expression of broiler chickens at 21 d of age (P < 0.05). Meanwhile, when considering Clostridium perfringens as the main effect, it also decreased the mRNA expression of the glucose transporter recombinant sodium/glucose cotransporter 1 (SGLT1) at d 21 and the fatty acid transporter liver fatty acid-binding protein (L-FABP) at d 42 (P < 0.05) as well as affect cecum microbial diversity, especially in relative abundance of Firmicutes and Bacteroidetes. In addition, Clostridium perfringens infection reduced body weight, daily weight gain, and feed-gain ratio (FCR) in broilers at d 42 (P < 0.05). The dietary β-1,3-glucan could alleviate intestinal mucosal damage caused by the Clostridium perfringens to some extent. When considering β-1,3-glucan as the main effect, it increased the SGLT1 at 42 d of age (P < 0.05), and stabilized gut microbiota disorder caused by Clostridium perfringens. More over dietary β-1,3-glucan addition increased body weight at 42-day-old (P < 0.05), and improved daily weight gain and FCR during 1 to 42 d (P < 0.05). In conclusion, dietary β-1,3-glucan could improve growth performance and intestinal health in broilers infected with Clostridium perfringens.