Response of the cricothyroid and thyroarytenoid muscles to stereotactic injection of substance P into the region of the nucleus tractus solitarius in developing dogs.
Response of the cricothyroid and thyroarytenoid muscles to stereotactic injection of substance P into the region of the nucleus tractus solitarius in developing dogs.
复制标题
将 P 物质立体定向注射到发育中的狗的孤束核区域，环甲腺和甲杓肌的反应。
DOI：
10.1177/000348940010901213
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Luschei,ES
中科院分区：
文献类型：
--
作者：
Bauman,NM;Wang,D;Sandler,AD;Luschei,ES
P物质（SP），一种公认的感觉神经递质，介导发育中狗的反射性喉内收肌活动。这种反射活动包括由刺激远端食管传入神经引起的危及生命的喉痉挛。SP的活动地点不明。本研究旨在探讨将SP注入发育中的比格犬孤束核（NTS）是否会改变喉内收肌的运动活动。6只动物，57 - 78日龄，接受5 - 10 μL SP立体定向注射到NTS区域，通过电刺激同侧上级喉神经识别。在另外8项研究中，将SP注射到远离NTS的小脑（2）或脑干（6）中。在所有6只动物中记录膈肌和环甲肌（CT）和/或甲杓肌（TA）的心血管和肌电图（EMG）反应。将SP注射到NTS区域中诱导所有动物的血压降低和同侧CT或TA活性的增加。其中3只动物出现混合性呼吸暂停，其特征为同侧CT或TA肌肉的持续EMG活动（痉挛）和无膈肌EMG活动。其中1只动物的呼吸暂停事件是致死性的。在6只接受脑干注射但在NTS区域外注射的动物中，注射SP后，膈肌和喉EMG活动通常没有变化，但1只动物的同侧CT活动出现轻度、短暂增加。在接受SP注射到小脑的两只动物中，均观察到同侧CT活动的短暂阶段性增加。一种假定的感觉神经递质，SP引起同侧CT和/或TA EMG活动时，注射到该地区的NTS在发展中的比格犬。这项研究表明，SP在NTS中可能发挥作用，在发展中的哺乳动物介导危及生命的喉内收肌反射，并可能提供有关治疗干预的重要信息。
Substance P (SP), a putative sensory neurotransmitter, mediates reflex laryngeal adductor activity in developing dogs. Such reflex activity includes life-threatening laryngospasm induced by stimulation of distal esophageal afferent nerves. The site of SP's activity is unknown. This research was undertaken to determine whether injection of SP into the nucleus tractus solitarius (NTS) of developing beagles alters laryngeal adductor motor activity. Six animals, 57 to 78 days of age, underwent stereotactic injection of 5 to 10 μL of SP into the region of the NTS, identified by electrical stimulation of the ipsilateral superior laryngeal nerve. In 8 additional studies, SP was injected into the cerebellum (2) or brain stem (6) distant from the NTS. Cardiovascular and electromyographic (EMG) responses of the diaphragm and the cricothyroid (CT) and/or thyroarytenoid (TA) muscles were recorded in all 6 animals. Injection of SP into the region of the NTS induced a decrease in blood pressure in all animals and an increase in either ipsilateral CT or TA activity. Three of these animals experienced mixed apnea characterized by sustained EMG activity (spasm) of the ipsilateral CT or TA muscles and an absence of diaphragm EMG activity. The apnea event was fatal in 1 of these animals. In the 6 animals who underwent injections in the brain stem but outside the region of the NTS, diaphragm and laryngeal EMG activity generally did not change after injection of SP, with the exception of 1 animal who experienced a mild, short-lived increase in ipsilateral CT activity. A brief phasic increase in ipsilateral CT activity was seen in both animals who underwent injection of SP into the cerebellum. A putative sensory neurotransmitter, SP evokes ipsilateral CT and/or TA EMG activity when injected into the region of the NTS in developing beagle dogs. This research suggests that SP in the NTS may play a role in mediating life-threatening laryngeal adductor reflexes in developing mammals and may provide important information regarding therapeutic intervention.