Crosstalk between elicitor-induced cell death and cell cycle regulation in tobacco BY-2 cells
Crosstalk between elicitor-induced cell death and cell cycle regulation in tobacco BY-2 cells
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DOI：
10.1111/j.1365-313x.2004.02197.x
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发表时间：
2004-10-01
期刊：
影响因子：
7.2
通讯作者：
Kuchitsu, K
中科院分区：
文献类型：
--
作者：
Kadota, Y;Watanabe, T;Kuchitsu, K
在植物中，细胞周期控制和程序性细胞死亡的调节之间的分子联系在很大程度上是未知的。本文以同步化烟草BY-2细胞为材料，研究了细胞周期与诱导子诱导的细胞死亡之间的关系。细胞核DNA的流式细胞术和荧光显微镜，和细胞周期相关基因的RNA凝胶印迹分析表明，蛋白质诱导剂隐地蛋白诱导细胞周期停滞在G1或G2期诱导细胞死亡之前。此外，在细胞周期的不同阶段，细胞死亡诱导和防御相关基因的模式是不同的。隐地蛋白的组成性治疗诱导细胞周期停滞和细胞死亡在G1或G2期。短暂处理2小时，细胞周期阻滞和细胞死亡，只有诱导剂处理期间的S或G1期。相比之下，诱导剂诱导的活性氧的产生过程中观察到的细胞周期的所有阶段。这些结果表明，虽然识别的激发子信号是细胞周期无关的，诱导细胞周期停滞和细胞死亡取决于细胞周期的阶段。
The molecular links between cell cycle control and the regulation of programmed cell death are largely unknown in plants. Here we studied the relationship between the cell cycle and elicitor-induced cell death using synchronized tobacco BY-2 cells. Flow cytometry and fluorescence microscopy of nuclear DNA, and RNA gel-blot analyses of cell cycle-related genes revealed that the proteinaceous elicitor cryptogein induced cell cycle arrest at the G1 or G2 phase before the induction of cell death. Furthermore, the patterns of cell death induction and defence-related genes were different in different phases of the cell cycle. Constitutive treatment with cryptogein induced cell cycle arrest and cell death at the G1 or G2 phase. With transient treatment for 2 h, cell cycle arrest and cell death were only induced by treatment with the elicitor during the S or G1 phase. By contrast, the elicitor-induced production of reactive oxygen species was observed during all phases of the cell cycle. These results indicate that although recognition of the elicitor signal is cell cycle-independent, the induction of cell cycle arrest and cell death depends on the phase of the cell cycle.