NERVE GROWTH-FACTOR INCREASES CHOLINE-ACETYLTRANSFERASE ACTIVITY IN DEVELOPING BASAL FOREBRAIN NEURONS
NERVE GROWTH-FACTOR INCREASES CHOLINE-ACETYLTRANSFERASE ACTIVITY IN DEVELOPING BASAL FOREBRAIN NEURONS
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DOI：
10.1016/0169-328x(86)90020-3
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发表时间：
1986-07-01
期刊：
影响因子：
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通讯作者：
JOHNSTON, MV
中科院分区：
文献类型：
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作者：
MOBLEY, WC;RUTKOWSKI, JL;JOHNSTON, MV
神经生长因子（NGF）是一种神经营养蛋白。其对发育中的外周感觉和交感神经元的作用已被广泛表征，但尚不清楚NGF是否在中枢神经系统神经元的发育过程中起作用。为了解决这一点，我们研究了神经生长因子对几个脑区神经递质酶活性的影响。脑室注射高纯度的小鼠神经生长因子对胆碱乙酰转移酶（ChAT）的活性有显着的影响，ChAT是胆碱能神经元的选择性标记。神经生长因子引起的启动子增加ChAT活性在基底前脑的新生大鼠，包括隔膜和一个区域，其中包含神经元的基底核和无名质。神经生长因子也增加了ChAT活性在海马和新皮质，终端地区的纤维基底前脑胆碱能神经元。与发育中的外周神经元的反应类似，NBF效应对基底前脑胆碱能细胞具有选择性，并且具有剂量依赖性。此外，隔神经元密切类似于交感神经元的时间过程中，他们对NGF的反应。这些观察结果表明，内源性神经生长因子发挥作用，基底前脑胆碱能神经元的发展。
Nerve growth factor (NGF) is a neuronotrophic protein. Its effects on developing peripheral sensory and sympathetic neurons have been extensively characterized, but it is not clear whether NGF plays a role during the development of central nervous system neurons. To address this point, we examined the effect of NGF on the activity of neurotransmitter enzymes in several brain regions. Intracerebroventricular injections of highly purified mouse NGF had a marked effect on the activity of choline acetyltransferase (ChAT), a selective marker of cholinergic neurons. NGF elicited promoter increases in ChAT activity in the basal forebrain of neonatal rats, including the septum and a region which contains neurons of the nucleus basalis and substantia innominata. NGF also increased ChAT activity in the hippocampus and neocortex, terminal regions for the fibers of basal forebrain cholinergic neurons. In analogy with the response of developing peripheral neurons, the NBF effect was shown to be selective for basal forebrain cholinergic cells and to be dose-dependent. Furthermore, septal neurons closely resembled sympathetic neurons in the time course of their response to NGF. These observations suggest that endogenous NGF does play a role in the development of basal forebrain cholinergic neurons.