Comparison of hyper-thermophilic-mesophilic two-stage with single-stage mesophilic anaerobic digestion of waste activated sludge: Process performance and microbial community analysis
Comparison of hyper-thermophilic-mesophilic two-stage with single-stage mesophilic anaerobic digestion of waste activated sludge: Process performance and microbial community analysis
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DOI：
10.1016/j.cej.2016.01.067
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发表时间：
2016-04
影响因子：
15.1
通讯作者：
Li-Jie Wu;Atsushi Higashimori;Yu Qin;Toshimasa Hojo;K. Kubota;Y. Li
中科院分区：
文献类型：
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作者：
Li-Jie Wu;Atsushi Higashimori;Yu Qin;Toshimasa Hojo;K. Kubota;Y. Li
新的污水处理工艺对传统的中温消化法处理活性污泥提出了挑战。采用高温(70℃)-中温(35℃)温控两段式无回流和有回流两级系统对废弃活性污泥的中温消化进行了升级研究。在两级系统中，固体还原提高了10%以上。在单级系统和两级系统中也观察到了类似的甲烷产量，甲烷产率为0.24g L/g VS。在没有回流的两级系统中，超高温阶段对COD的溶解起着重要作用，该阶段溶解了34.4%的COD，而中温阶段对甲烷产生的贡献更大，其中36.2%的COD在中温阶段以甲烷的形式产生。与单级系统中的原生细菌相比，微生物成为两级系统中嗜热阶段中最优势的细菌门，在超高温阶段以红衣藻为优势菌。两级系统的循环增加了超高温阶段微生物群落的多样性，这是导致超高温阶段甲烷产量提高的因素之一。
The conventional mesophilic digestion of waste activated sludge (WAS) is challenged by the updated wastewater treatment process. A hyper-thermophilic (70 °C)–mesophilic (35 °C) temperature-phased two-stage systems without and with recirculation were employed to upgrade the single-stage mesophilic digestion of waste activated sludge in this study. The solid reduction increased by more than 10% in the two-stage systems. Similar methane production was observed in the single-stage system and two-stage systems, with a methane yield of 0.24 L/g VS added. In the case of two-stage system without recirculation, hyper-thermophilic stage played an important role in COD solubilization, with 34.4% of the WAS solubilized in that stage, while mesophilic stage contributed more in methane production, with 36.2% of COD in the WAS produced as methane in the mesophilic stage.Firmicutesbecame the most dominant bacteria phylum in the mesophilic stages of two-stage systems as compared to the dominant phylumProteobacteriain single-stage system, with the genusMethanothermobactercardinal in the hyper-thermophilic stage. The recirculation in the two-stage system increased the microbial community diversity in the hyper-thermophilic stage, which was one factor to result in improved methane production in the hyper-thermophilic stage.