The upregulation of hypoxia-related miRNA 210 in primary tumor of lymphogenic metastatic prostate cancer
The upregulation of hypoxia-related miRNA 210 in primary tumor of lymphogenic metastatic prostate cancer
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DOI：
10.2217/epi-2017-0114
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发表时间：
2018-10-01
期刊：
影响因子：
3.8
通讯作者：
Warnecke-Eberz, Ute
中科院分区：
文献类型：
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作者：
Eminaga, Okyaz;Fries, Jochen;Warnecke-Eberz, Ute
目的：探讨hsa-miR-210（miR-210）在前列腺癌（PCa）中的表达水平与肿瘤进展的关系。研究方法：对55名不同肿瘤分期的受试者进行定量PCR检测miR-210;然后使用三个外部数据集验证我们的结果。进行ANOVA和Tukey事后分析，用于不同肿瘤阶段之间的比较分析。使用来自The Cancer Genome Atlas for CaP的转录组数据，对在Tarbase和miRWalk数据集中鉴定的实验验证的miR-210靶基因进行基因表达分析。结果与结论：与非转移性PCa相比，miR-210在N1 PCa中显著更高，而转移性肿瘤显示miR-210的表达水平低于原发性肿瘤。
Aim: To show the association between the expression level of hsa-miR-210 (miR-210) and tumor progression in prostate cancer (PCa). Methods: Quantitative PCR was performed to measure miR-210 on 55 subjects with different tumor stages; our results were then validated using three external datasets. ANOVA and Tukey's post hoc analysis were performed for comparative analyses between different tumor stages. Using the transcriptome data from The Cancer Genome Atlas for CaP, the gene expression analyses were performed on experimentally validated target genes of miR-210 identified in Tarbase and miRWalk datasets. Results & conclusion: miR-210 was significantly higher in N1 PCa compared with nonmetastatic PCa, whereas the metastatic tumor revealed a lower expression level of miR-210 than the primary tumor.