Addition of p-azido-L-phenylaianine to the genetic code of Escherichia coli
Addition of p-azido-L-phenylaianine to the genetic code of Escherichia coli
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DOI：
10.1021/ja027007w
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发表时间：
2002-08-07
影响因子：
15
通讯作者：
Schultz, PG
中科院分区：
文献类型：
--
作者：
Chin, JW;Santoro, SW;Schultz, PG
We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker,p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.
