Molecular cloning, expression profiles, and characterization of a novel polyphenol oxidase (PPO) gene in Hevea brasiliensis
Molecular cloning, expression profiles, and characterization of a novel polyphenol oxidase (PPO) gene in Hevea brasiliensis
复制标题
巴西橡胶树中新型多酚氧化酶 (PPO) 基因的分子克隆、表达谱和表征
DOI：
10.1080/09168451.2014.940828
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发表时间：
2014-10-03
影响因子：
1.6
通讯作者：
Liu, Hui
中科院分区：
文献类型：
--
作者：
Li, Dejun;Deng, Zhi;Liu, Hui
多酚氧化酶（PPO）参与许多植物性食品中的不良布朗宁。虽然已有研究者对橡胶多酚氧化酶进行了研究，但橡胶多酚氧化酶基因的分离和表达谱的研究尚未见报道。本研究从橡胶树中分离到一个新的多酚氧化酶基因HbPPO。序列比对结果表明，HbPPO与植物PPO具有较高的同源性，属于双子叶植物的分支。预测了HbPPO启动子区存在与胁迫/激素反应相关的顺式调控元件。实时荧光定量RT-PCR分析表明，HbPPO的表达量变化很大，这取决于不同的组织和发育阶段的叶片。除了与割胶板干燥，HbPPO转录调节乙烯利，创伤，过氧化氢，茉莉酸甲酯治疗。此外，本研究还进一步证实了胶乳凝固速率与PPO活性之间的相关性。研究结果为进一步分析橡胶树中HbPPO的功能奠定了基础。
The polyphenol oxidase (PPO) is involved in undesirable browning in many plant foods. Although the PPOs have been studied by several researchers, the isolation and expression profiles of PPO gene were not reported in rubber tree. In this study, a new PPO gene, HbPPO, was isolated from Hevea brasiliensis. The sequence alignment showed that HbPPO indicated high identities to plant PPOs and belonged to dicot branch. The cis-acting regulatory elements related to stress/hormone responses were predicted in the promoter region of HbPPO. Real-time RT-PCR analyses showed that HbPPO expression varied widely depending on different tissues and developmental stages of leaves. Besides being associated with tapping panel dryness, the HbPPO transcripts were regulated by ethrel, wounding, H2O2, and methyl jasmonate treatments. Moreover, the correlation between latex coagulation rate and PPO activity was further confirmed in this study. Our results lay the foundation for further analyzing the function of HbPPO in rubber tree.