ANALYSIS OF PROVIRAL INTEGRATION IN HUMAN MAMMARY EPITHELIAL-CELL LINES IMMORTALIZED BY RETROVIRAL INFECTION WITH A TEMPERATURE-SENSITIVE SV40 T-ANTIGEN CONSTRUCT
ANALYSIS OF PROVIRAL INTEGRATION IN HUMAN MAMMARY EPITHELIAL-CELL LINES IMMORTALIZED BY RETROVIRAL INFECTION WITH A TEMPERATURE-SENSITIVE SV40 T-ANTIGEN CONSTRUCT
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DOI：
10.1002/ijc.2910570616
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发表时间：
1994-06-15
影响因子：
6.4
通讯作者：
OHARE, MJ
中科院分区：
文献类型：
--
作者：
STAMPS, AC;DAVIES, SC;OHARE, MJ
一组8个条件永生细胞系的人乳腺上皮细胞感染嗜中性逆转录病毒转导的SV 40大T-抗原的TS突变体进行了分析，相对于个人的逆转录病毒整合模式。每个细胞系含有多个整合位点，这些整合位点是克隆的并且在延长的传代中是稳定的。类似的整合模式之间观察到的个别线分别从同一股票的前永生细胞，这表明一个共同的祖先。在危机前生长的不同阶段的前永生细胞的逆转录病毒整合分析表明，随着细胞接近危机，从多克隆性到克隆性的逐步过渡的变化。每一条仙线都有一个准仙祖线的融合点，每一个融合点都有自己的组合和复制数量。由于所有的细胞系似乎都起源于单独培养瓶中的单个病灶，因此很可能每一组都是由不同遗传事件导致的永生前细胞的共同克隆产生的。从这个分析中没有证据表明，特定的融合点在危机前克隆人的选择或随后建立不朽系中发挥了任何作用。(C)1994 Wiley-Liss，Inc.
A panel of eight conditionally immortal lines derived by infection of human breast epithelial cells with an amphotropic retrovirus transducing a ts mutant of SV40 large T-antigen was analyzed with respect to individual retroviral integration patterns. Each line contained multiple integration sites which were clonal and stable over extended passage. Similar integration patterns were observed between individual lines arising separately from the same stock of pre-immortal cells, suggesting a common progenitor. Retroviral integration analysis of pre-immortal cells at different stages of pre-crisis growth showed changes indicative of a progressive transition from polyclonality to clonality as the cells approached crisis. Each of the immortal lines contained a sub-set of the integration sites of their pre-immortal progenitors, with individual combinations and copy numbers of sites. Since all the cell lines appeared to originate from single foci in separate flasks, it is likely that each set arose from a common clone of pre-immortal cells as the result of separate genetic events. There was no evidence from this analysis to suggest that specific integration sites played any part either in the selection of pre-crisis clones or in the subsequent establishment of immortal lines. (C) 1994 Wiley-Liss, Inc.