Gas chromatographic determination of bupivacaine in plasma using a support coated open tubular column and a nitrogen-selective detector.
Gas chromatographic determination of bupivacaine in plasma using a support coated open tubular column and a nitrogen-selective detector.
复制标题
使用支撑涂层开管柱和氮选择性检测器对血浆中的布比卡因进行气相色谱测定。
作者：
A. Burm;J. V. van Kleef;A. D. de Boer
建立了毛细管气相色谱测定血浆中布比卡因浓度的方法。用正戊烷从血浆中提取布比卡因和添加的内标。提取的产物通过固相进样系统进入色谱仪。载体涂层开管柱和氮选择性检测器的组合确保了良好的选择性和高灵敏度。浓度超过3 ng布比卡因/毫升血浆时，变异系数小于6%。检测下限约为1 ng/ml。
A capillary gas chromatographic method for the quantitative determination of bupivacaine in plasma is described. Bupivacaine and an added internal standard were extracted from plasma with n-pentane. The extracted products were introduced into the gas chromatograph via a solid injection system. The combination of a support coated open tubular column and a nitrogen-selective detector insure a good selectivity and a high sensitivity. The coëfficient of variation at concentrations exceeding 3 ng bupivacaine/ml plasma is less than 6%. The detection limit is about 1 ng/ml.