Evaluation of EpiDerm full thickness-300 (EFT-300) as an in vitro model for skin irritation: Studies on aliphatic hydrocarbons
Evaluation of EpiDerm full thickness-300 (EFT-300) as an in vitro model for skin irritation: Studies on aliphatic hydrocarbons
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DOI：
10.1016/j.tiv.2009.08.019
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发表时间：
2010-03-01
影响因子：
3.2
通讯作者：
Singh, Mandip S.
中科院分区：
文献类型：
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作者：
Mallampati, Ramya;Patlolla, Ram R.;Singh, Mandip S.
本研究的目的是利用体外三维表皮全厚度-300 （EFT-300）皮肤培养物，了解饱和脂肪烃（HCs） C9-C16对皮肤的刺激作用。用2.5 μ l的hc处理EFT-300培养物，在24和48 h收集培养基和皮肤样本，测量各种炎症生物标志物（IL-1 α， IL-6和IL-8）的释放。为了验证体外结果，在无毛大鼠中进行了体内皮肤刺激研究，通过测量非闭合的hc皮肤暴露72小时后的表皮水分流失（TEWL）和红斑。EFT-300组织的MTT组织活力测定结果显示，2.5 μ l/组织（约4.1 μ l/cm(2)）的hc在暴露48小时内不会引起组织活力的任何显着变化。电镜观察EFT-300的横截面，24 h时样品的组织形态没有明显变化，但暴露48 h后，十三烷、十四烷和十六烷对角质层产生轻微的增厚和破坏。C12-C16 hc暴露于皮肤24小时后，IL-1 α在皮肤和培养基中的表达显著增加。同样，所有HC暴露于皮肤24小时后，皮肤和培养基中白细胞介素-6 （IL-6）和IL-8的表达与HC链长度成比例显著增加。随着暴露时间增加到48小时，IL-6浓度比24小时时的IL-6浓度增加了2倍。体内皮肤刺激数据还显示，TEWL和红斑评分随着hc链长度的增加而增加（C9-C16）。综上所述，EFT-300显示hc对皮肤的刺激程度为C9级
The aim of this study was to understand the skin irritation effects of saturated aliphatic hydrocarbons (HCs), C9-C16, found jet fuels using in vitro 3-dimensional EpiDerm full thickness-300 (EFT-300) skin cultures. The EFT-300 cultures were treated with 2.5 mu l of HCs and the culture medium and skin samples were collected at 24 and 48 h to measure the release of various inflammatory biomarkers (IL-1 alpha, IL-6 and IL-8). To validate the in vitro results, in vivo skin irritation studies were carried out in hairless rats by measuring trans epidermal water loss (TEWL) and erythema following un-occlusive dermal exposure of HCs for 72 h. The MTT tissue viability assay results with the EFT-300 tissue show that 2.5 mu l/tissue (approximate to 4.1 mu l/cm(2)) of the HCs did not induce any significant changes in the tissue viability for exposure times up to 48 h of exposure. Microscopic observation of the EFT-300 cross-sections indicated that there were no obvious changes in the tissue morphology of the samples at 24 h, but after 48 h of exposure, tridecane, tetradecane and hexadecane produced a slight thickening and disruption of stratum corneum. Dermal exposures of C12-C16 HCs for 24 h significantly increased the expression of IL-1 alpha in the skin as well as in the culture medium. Similarly, dermal exposure of all HCs for 24 h significantly increased the expression of interleukin-6 (IL-6) and IL-8 in the skin as well as in the culture medium in proportion to the HC chain length. As the exposure time increased to 48 h. IL-6 concentrations increased 2-fold compared to the IL-6 values at 24 h. The in vivo skin irritation data also showed that both TEWL and erythema scores increased with increased HCs chain length (C9-C16). In conclusion, the EFT-300 showed that the skin irritation profile of HCs was in the order of C9