Expression and potential role of dentin phosphophoryn (DPP) in mouse embryonic tissues involved in epithelial-mesenchymal interactions and branching morphogenesis
Expression and potential role of dentin phosphophoryn (DPP) in mouse embryonic tissues involved in epithelial-mesenchymal interactions and branching morphogenesis
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DOI：
10.1002/dvdy.20935
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发表时间：
2006-11-01
影响因子：
2.5
通讯作者：
Veis, Arthur
中科院分区：
文献类型：
--
作者：
Alvares, Keith;Kanwar, Yashpal S.;Veis, Arthur
牙本质涎磷蛋白(DSPP)是在牙齿发育的不同阶段，在间充质和上皮细胞中合成的。在牙帽期，对应于小鼠胚胎发育的13.5天，DSPP的磷酸(DPP)部分免疫组织化学定位于釉器，在口腔外胚层有较强的染色，而在牙囊间充质中无表达。令人惊讶的是，DPP也在胚胎后肾的输尿管芽支和发育中的肺的肺泡上皮芽中表达。逆转录聚合酶链式反应分析证实，在牙齿、肺和肾脏中存在序列相同的DSPP mRNA。DSPP-/-小鼠在牙齿中表达DPP，在肾脏和肺中也表现出器官发生的异常。在肾脏中，可见畸形的后肾S小体和间充质细胞凋亡增加。在E13.5野生型小鼠的后肾器官培养中加入抗DPP抗体，同样导致输尿管芽形态发生改变，表明DPP在胚胎发育过程中分生组织中的上皮-间充质相互作用中发挥作用。
Dentin sialophosphoprotein (DSPP) is synthesized in both mesenchyme and epithelium at varying stages of tooth development. At the tooth cap stage, corresponding to embryonic day (E) 13.5 of mouse embryonic life, the phosphophoryn (DPP) portion of DSPP was immunohistochemically localized to the enamel organ with intense staining of oral ectoderm but no expression in dental follicle mesenchyme. Surprisingly, DPP was also expressed in ureteric bud branches of embryonic metanephric kidney and alveolar epithelial buds of developing lung. Reverse transcriptase-polymerase chain reaction analysis verified the presence of DSPP mRNA with identical sequences in the tooth, lung, and kidney. The DSPP-/- mouse with ablated DPP expression in the teeth, also exhibited aberrant organogenesis in kidney and lung. In the kidney, malformed metanephric S-shaped bodies and increased mesenchymal apoptosis were observed. Inclusion of anti-DPP antibodies in organ culture of metanephroi, harvested from E13.5 wild-type mice, likewise resulted in altered ureteric bud morphogenesis, suggesting a role for DPP in epithelial-mesenchymal interactions in meristic tissues during embryonic development.